pcDNA3 Flag TSC2 | Addgene 14129 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3 Flag TSC2

catalog :

14129

citations: 16

Reference
Hao C, Sheng Z, Wang W, Feng R, Zheng Y, Xiao Q, et al. Tumor-derived exosomal miR-148b-3p mediates M2 macrophage polarization via TSC2/mTORC1 to promote breast cancer migration and invasion. Thorac Cancer. 2023;14:1477-1491 pubmed publisher
Teran O, Zanotelli M, Lin M, Cerione R, Wilson K. Dock7 regulates AKT and mTOR/S6K activity required for the transformed phenotypes and survival of cancer cells. bioRxiv. 2023;: pubmed publisher
Zhong Y, Zhou X, Guan K, Zhang J. Rheb regulates nuclear mTORC1 activity independent of farnesylation. Cell Chem Biol. 2022;29:1037-1045.e4 pubmed publisher
Zhao Y, Liang Y, Xu Z, Liu J, Liu X, Ma J, et al. Exosomal miR-673-5p from fibroblasts promotes Schwann cell-mediated peripheral neuron myelination by targeting the TSC2/mTORC1/SREBP2 axis. J Biol Chem. 2022;298:101718 pubmed publisher
Cui M, Jiang W, Sheng J, Wu J, Zhang X. Rap_GAP Domain of TSC2 Contributes to Tumor Suppression Through mTOR Signaling in Human Hepatocellular Carcinoma. DNA Cell Biol. 2022;41:215-224 pubmed publisher
Araki M, Yoshimoto K, Ohta M, Katada T, Kontani K. Development of a versatile HPLC-based method to evaluate the activation status of small GTPases. J Biol Chem. 2021;297:101428 pubmed publisher
Gu N, Li G, Yang B, You M, Lin Y, Sun F, et al. Hypo-Expression of Tuberin Promotes Adenomyosis via the mTOR1-Autophagy Axis. Front Cell Dev Biol. 2021;9:710407 pubmed publisher
Amemiya Y, Nakamura N, Ikeda N, Sugiyama R, Ishii C, Maki M, et al. Amino Acid-Mediated Intracellular Ca2+ Rise Modulates mTORC1 by Regulating the TSC2-Rheb Axis through Ca2+/Calmodulin. Int J Mol Sci. 2021;22: pubmed publisher
Romero Pozuelo J, Figlia G, Kaya O, Martin Villalba A, Teleman A. Cdk4 and Cdk6 Couple the Cell-Cycle Machinery to Cell Growth via mTORC1. Cell Rep. 2020;31:107504 pubmed publisher
Byun J, Park M, Yun J, Lee J, Kim J, Cho S, et al. Oncogenic KRAS signaling activates mTORC1 through COUP-TFII-mediated lactate production. EMBO Rep. 2019;20: pubmed publisher
Norambuena A, Wallrabe H, Cao R, Wang D, Silva A, Švindrych Z, et al. A novel lysosome-to-mitochondria signaling pathway disrupted by amyloid-β oligomers. EMBO J. 2018;37: pubmed publisher
Chiang Y, Liao W, Ho K, Wang S, Chen Y, Ho C, et al. CBAP modulates Akt-dependent TSC2 phosphorylation to promote Rheb-mTORC1 signaling and growth of T-cell acute lymphoblastic leukemia. Oncogene. 2019;38:1432-1447 pubmed publisher
Hao F, Kondo K, Itoh T, Ikari S, Nada S, Okada M, et al. Rheb localized on the Golgi membrane activates lysosome-localized mTORC1 at the Golgi-lysosome contact site. J Cell Sci. 2018;131: pubmed publisher
Henderson J, Wagner J, Hofmann N, Eide C, Cho Y, Druker B, et al. Functional validation of the oncogenic cooperativity and targeting potential of tuberous sclerosis mutation in medulloblastoma using a MYC-amplified model cell line. Pediatr Blood Cancer. 2017;64: pubmed publisher
Bento C, Ashkenazi A, Jimenez Sanchez M, Rubinsztein D. The Parkinson's disease-associated genes ATP13A2 and SYT11 regulate autophagy via a common pathway. Nat Commun. 2016;7:11803 pubmed publisher
Manning B, Tee A, Logsdon M, Blenis J, Cantley L. Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway. Mol Cell. 2002;10:151-62 pubmed

product information

Catalog Number :

14129

Product Name :

pcDNA3 Flag TSC2

article :

doi	10.1016/S1097-2765(02)00568-3
id	1260
pubmed_id	12150915

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3-Flag

backbone_mutation

backbone_origin

FLAG (MDYKDDDDK) was cloned between HindIII and BamHI of pcDNA3

backbone_size

5500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The first 276 base pairs of TSC2 were PCR-amplified from pcDNA6-TSC2 and inserted between the BamHI and NotI sites in frame with the FLAG-coding sequence in pcDNA3-FLAG. The remainder of TSC2 was subcloned from pcDNA6-TSC2 and inserted between the NotI and XbaI sites of the above construct. Diagnostic digests reveal an extra BamHI site in this plasmid. BamHI will not linearize the construct.

origin :

pi :

alt_names

tuberin

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	BamHI
promoter
sequencing_primer_3	SP6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	LAM, PPP1R160, TSC4
gene	TSC2
id	7249

genbank_ids

mutation

name

TSC2

shRNA_sequence

size

5400

species

9606

Homo sapiens