This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET20b-delta133HtrA2
catalog :
14126
citations: 2
Reference
Merski M, Moreira C, Abreu R, Ramos M, Fernandes P, Martins L, et al. Molecular motion regulates the activity of the Mitochondrial Serine Protease HtrA2. Cell Death Dis. 2017;8:e3119 pubmed publisher
Martins L, Turk B, Cowling V, Borg A, Jarrell E, Cantley L, et al. Binding specificity and regulation of the serine protease and PDZ domains of HtrA2/Omi. J Biol Chem. 2003;278:49417-27 pubmed
product information
Catalog Number :
14126
Product Name :
pET20b-delta133HtrA2
article :
doi10.1074/jbc.M308659200
id1255
pubmed_id14512424
bacterial resistance :
Ampicillin
cloning :
backbonepET20b
backbone_mutation
backbone_originNovagen
backbone_size3584
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth temp :
When transformed and plated on LB/agar/Amp plates, this plasmid will normally only produce colonies if the plates are incubated at room temp as opposed to 37oC. Following this, the bacteria when inoculated in liquid media will grow well at 37oC.
origin :
37
pi :
alt_names
Omi
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMGCA8, OMI, PARK13, PRSS25
geneHTRA2
id27429
genbank_ids
NM_013247
mutation
nameHtrA2
shRNA_sequence
size978
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHis
resistance markers :
311
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA