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product summary
company name :
Addgene
product type :
cDNA
product name :
pCEP4-myc-ACE2
catalog :
141185
citations: 29
Reference
Bernardotto S, Frasson I, Faravelli S, Morelli A, Schiavon E, Moscatiello G, et al. Efficient SARS-CoV-2 infection antagonization by rhACE2 ectodomain multimerized onto the Avidin-Nucleic-Acid-NanoASsembly. Biomaterials. 2023;303:122394 pubmed publisher
Seixas M, Bartolomeo C, Lemes R, Nicoliche T, Okuda L, Martins L, et al. Disruptive 3D in vitro models for respiratory disease investigation: A state-of-the-art approach focused on SARS-CoV-2 infection. Biomater Biosyst. 2023;11:100082 pubmed publisher
Wu C, Yang Y, Lin Y, Shu L, Cheng Y, Liu H, et al. The anti-SARS-CoV-2 effect and mechanism of Chiehyuan herbal oral protection solution. Heliyon. 2023;9:e17701 pubmed publisher
Colom M, Vucinic J, Adolf Bryfogle J, Bowman J, Verel S, Moczygemba I, et al. Deep Evolutionary Forecasting identifies highly-mutated SARS-CoV-2 variants via functional sequence-landscape enumeration. Res Sq. 2022;: pubmed publisher
Kim T, Kim J, Kwon H, Jeon S, Lee S, Jung H, et al. Astersaponin I from Aster koraiensis is a natural viral fusion blocker that inhibits the infection of SARS-CoV-2 variants and syncytium formation. Antiviral Res. 2022;208:105428 pubmed publisher
Park J, Park M, Kim Y, Jun Y, Lee U, Oh C. Apelin as a new therapeutic target for COVID-19 treatment. QJM. 2022;: pubmed publisher
Parker E, Anderson C, Zeller M, Tibi A, Havens J, Laroche G, et al. Regional connectivity drove bidirectional transmission of SARS-CoV-2 in the Middle East during travel restrictions. Nat Commun. 2022;13:4784 pubmed publisher
Mekhail K, Lee M, Sugiyama M, Astori A, St Germain J, Latreille E, et al. FASN inhibitor TVB-3166 prevents S-acylation of the spike protein of human coronaviruses. J Lipid Res. 2022;63:100256 pubmed publisher
Nagappan A, Kim K, Moon Y. Caveolin-1-ACE2 axis modulates xenobiotic metabolism-linked chemoresistance in ovarian clear cell carcinoma. Cell Biol Toxicol. 2022;: pubmed publisher
Muena N, García Salum T, Pardo Roa C, Avendaño M, Serrano E, Levican J, et al. Induction of SARS-CoV-2 neutralizing antibodies by CoronaVac and BNT162b2 vaccines in naïve and previously infected individuals. EBioMedicine. 2022;78:103972 pubmed publisher
Pinto A, Rai R, Brown J, Griffin P, Edgar J, Shah A, et al. Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium. Nat Commun. 2022;13:1609 pubmed publisher
Tsai M, Shih W, Yang Y, Lin Y, Chang G, Hsu C, et al. Potential inhibitor for blocking binding between ACE2 and SARS-CoV-2 spike protein with mutations. Biomed Pharmacother. 2022;149:112802 pubmed publisher
Ferrucci V, De Antonellis P, Quarantelli F, Asadzadeh F, Bibbò F, Siciliano R, et al. Loss of Detection of sgN Precedes Viral Abridged Replication in COVID-19-Affected Patients-A Target for SARS-CoV-2 Propagation. Int J Mol Sci. 2022;23: pubmed publisher
Zhang L, Dutta S, Xiong S, Chan M, Chan K, Fan T, et al. Engineered ACE2 decoy mitigates lung injury and death induced by SARS-CoV-2 variants. Nat Chem Biol. 2022;18:342-351 pubmed publisher
Grishin A, Dolgova N, Landreth S, Fisette O, Pickering I, George G, et al. Disulfide Bonds Play a Critical Role in the Structure and Function of the Receptor-binding Domain of the SARS-CoV-2 Spike Antigen. J Mol Biol. 2022;434:167357 pubmed publisher
Christodoulou I, Rahnama R, Ravich J, Seo J, Zolov S, Marple A, et al. Glycoprotein Targeted CAR-NK Cells for the Treatment of SARS-CoV-2 Infection. Front Immunol. 2021;12:763460 pubmed publisher
Zhang L, Dutta S, Xiong S, Chan M, Chan K, Fan T, et al. Engineered High-Affinity ACE2 Peptide Mitigates ARDS and Death Induced by Multiple SARS-CoV-2 Variants. bioRxiv. 2021;: pubmed publisher
Sansico F, Miroballo M, Bianco D, Tamiro F, Colucci M, Santis E, et al. COVID-19 Specific Immune Markers Revealed by Single Cell Phenotypic Profiling. Biomedicines. 2021;9: pubmed publisher
Durdagi S, Avsar T, Orhan M, Serhatlı M, Balcioglu B, Ozturk H, et al. The neutralization effect of montelukaston SARS-CoV-2 is shown by multiscale in silicosimulations and combined in vitro studies. Mol Ther. 2021;: pubmed publisher
Dogan K, Erol E, Didem Orhan M, Degirmenci Z, Kan T, Gungor A, et al. Instant determination of the artemisinin from various Artemisia annua L. extracts by LC-ESI-MS/MS and their in-silico modelling and in vitro antiviral activity studies against SARS-CoV-2. Phytochem Anal. 2021;: pubmed publisher
Havranek B, Chan K, Wu A, Procko E, Islam S. Computationally Designed ACE2 Decoy Receptor Binds SARS-CoV-2 Spike (S) Protein with Tight Nanomolar Affinity. J Chem Inf Model. 2021;61:4656-4669 pubmed publisher
Tsai M, Yang Y, Lin Y, Chang G, Hsu C, Yeh R, et al. GB-2 blocking the interaction between ACE2 and wild type and mutation of spike protein of SARS-CoV-2. Biomed Pharmacother. 2021;142:112011 pubmed publisher
Cecon E, Burridge M, Cao L, Carter L, Ravichandran R, Dam J, et al. SARS-COV-2 spike binding to ACE2 in living cells monitored by TR-FRET. Cell Chem Biol. 2021;: pubmed publisher
Zhang Q, Gefter J, Sneddon W, Mamonova T, Friedman P. ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion. iScience. 2021;24:102770 pubmed publisher
Kim T, Jeon S, Jang Y, Gotina L, Won J, Ju Y, et al. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med. 2021;53:956-972 pubmed publisher
Laffeber C, de Koning K, Kanaar R, Lebbink J. Experimental Evidence for Enhanced Receptor Binding by Rapidly Spreading SARS-CoV-2 Variants. J Mol Biol. 2021;433:167058 pubmed publisher
Suh J, Kim H, Kim T. Development of a SARS-CoV-2-derived receptor-binding domain-based ACE2 biosensor. Sens Actuators B Chem. 2021;334:129663 pubmed publisher
Chan K, Dorosky D, Sharma P, Abbasi S, Dye J, Kranz D, et al. Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2. Science. 2020;369:1261-1265 pubmed publisher
Procko E. The sequence of human ACE2 is suboptimal for binding the S spike protein of SARS coronavirus 2. bioRxiv. 2020;: pubmed publisher
product information
Catalog Number :
141185
Product Name :
pCEP4-myc-ACE2
article :
doi10.1126/science.abc0870
id28211097
pubmed_id32753553
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutation
backbone_originInvitrogen
backbone_size10381
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/2020.03.16.994236v1 for BioRxiv preprint.
growth strain :
Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag
origin :
37
pi :
alt_names
ACE2
peptidyl-dipeptidase A
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NheI
promoterCMV
sequencing_primer_3EBV-rev
sequencing_primer_5CMV-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACEH
geneACE2
id59272
genbank_ids
NM_021804.1
mutation
nameAngiotensin-converting enzyme 2
shRNA_sequence
size2481
species
9606
Homo sapiens
tags
locationN terminal on insert
tagSignal peptide from influenza HA
locationN terminal on insert
tagExtracellular c-myc epitope tag
resistance markers :
3492
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA