This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RA35
catalog :
141126
citations: 1
Reference
Alnahhas R, Sadeghpour M, Chen Y, Frey A, Ott W, Josic K, et al. Majority sensing in synthetic microbial consortia. Nat Commun. 2020;11:3659 pubmed publisher
product information
Catalog Number :
141126
Product Name :
RA35
article :
doi10.1038/s41467-020-17475-z
id28211082
pubmed_id32694598
bacterial resistance :
Chloramphenicol
cloning :
backbonepSC101
backbone_mutationspacer sequence between two inserts
backbone_origin
backbone_size6484
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses dimeric LacI repressor (LacI-11) under Rhl promoter
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterengineered rhl promoter (responds to C4-HSL)
sequencing_primer_3TTGCGGGCAACTTCAGCA
sequencing_primer_5TCCCAACCTTACCAGAGGGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0345, ECK0342
genelacI
id945007
genbank_ids
mutationtruncated/dimeric version of LacI (missing last 11 amino acids = tetramerization domain)
namedimeric LacI (LacI-11)
shRNA_sequence
size1047
species
100
Synthetic
tags
locationC terminal on backbone
tagssrA degradation tag
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterengineered xyl promoter (responds to xylose)
sequencing_primer_3TAGTGACCTGTTCGTTGCAACA
sequencing_primer_5gaacggtttcactaggaagcaag
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAQ980_RS12585, AQ980_12820
geneAQ980_RS12585
id31629115
genbank_ids
mutation
nameAiiA
shRNA_sequence
size749
species
tags
locationC terminal on backbone
tagssrA degradation tag
resistance markers :
1988
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA