This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)
catalog :
140893
citations: 1
Reference
Kawanabe A, Mizutani N, Polat O, Yonezawa T, Kawai T, Mori M, et al. Engineering an enhanced voltage-sensing phosphatase. J Gen Physiol. 2020;152: pubmed publisher
product information
Catalog Number :
140893
Product Name :
CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)
article :
doi10.1085/jgp.201912491
id28211028
pubmed_id32167537
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(-)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5XhoI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
genevsp
id619223
aliasestpip, vsp, wu:fd20e11, wu:fi24b06
genetpte
id327292
genbank_ids
NM_001025458.1
NM_001033826.1
mutationchanged 223L to F in Dr-VSP (zebrafish TPTE), changed 302C to S in Dr-VSP (zebrafish TPTE)
nameCiDr-VSP L223F/C302S-mCherry
shRNA_sequence
size2433
species
7955
Danio rerio
32644
Other
Ciona intestinalis
tags
location
tagmCherry
resistance markers :
2949
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA