This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FUG-T2A-D252G
catalog :
140741
citations: 1
Reference
Fricano Kugler C, Getz S, Williams M, Zurawel A, DeSpenza T, Frazel P, et al. Nuclear Excluded Autism-Associated Phosphatase and Tensin Homolog Mutations Dysregulate Neuronal Growth. Biol Psychiatry. 2018;84:265-277 pubmed publisher
product information
Catalog Number :
140741
Product Name :
FUG-T2A-D252G
article :
doi10.1016/j.biopsych.2017.11.025
id28211008
pubmed_id29373119
bacterial resistance :
Ampicillin
cloning :
GTACTtAGGGCCGGGATTCTCCTCCACGTCACCGCATGTTAGAAGACTTCCTCTGCCCTCCTTbackbone_originbackbone_size9248promotersequencing_primer_3sequencing_primer_5vector_types
Mammalian Expression
Lentiviral

GTACAAGGAGGGCAGAGGAAGTCTTCTAACATGCGGTGA
CGTGGAGGAGAATCCCGGCCCTaA
backboneFUG-PtenD252G
backbone_mutationThe following oligos were annealed and cloned into the BsrG1 site of GFP to generate a T2A linker between the GFP-Pten.
growth strain :
Lentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteins
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsrG1
cloning_site_5BsrG1
promoterhUbiC
sequencing_primer_3WRE up
sequencing_primer_5GFP Down
site_3_destroyed1
site_5_destroyed
entrez_gene
aliases10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1
genePTEN
id5728
genbank_ids
mutationD252G
nameGFP-T2A-PTEND252G
shRNA_sequence
size1998
species
9606
Homo sapiens
tags
location
tagnone
plasmid copy :
Pten insert from Alonzo Ross
resistance markers :
2405
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA