pCMV5 DPC4 (1-514) | Addgene 14040 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV5 DPC4 (1-514)

catalog :

14040

citations: 3

Reference
Mazzocchi M, Wyatt S, Mercatelli D, Morari M, Morales Prieto N, Collins L, et al. Gene Co-expression Analysis Identifies Histone Deacetylase 5 and 9 Expression in Midbrain Dopamine Neurons and as Regulators of Neurite Growth via Bone Morphogenetic Protein Signaling. Front Cell Dev Biol. 2019;7:191 pubmed publisher
Tatler A, Goodwin A, Gbolahan O, Saini G, Porte J, John A, et al. Amplification of TGF? Induced ITGB6 Gene Transcription May Promote Pulmonary Fibrosis. PLoS ONE. 2016;11:e0158047 pubmed publisher
Lagna G, Hata A, Hemmati Brivanlou A, Massague J. Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways. Nature. 1996;383:832-6 pubmed

product information

Catalog Number :

14040

Product Name :

pCMV5 DPC4 (1-514)

article :

doi	10.1038/383832a0
id	1218
pubmed_id	8893010

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV5

backbone_mutation

backbone_origin

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Dominant negative mutant of DPC4. See article and author's map for more information.

origin :

pi :

alt_names

DPC4

cloning

clone_method	Restriction Enzyme
cloning_site_3	BamHI
cloning_site_5	HindIII
promoter
sequencing_primer_3
sequencing_primer_5	CMV Forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DPC4, JIP, MADH4, MYHRS
gene	SMAD4
id	4089

genbank_ids

mutation

R515 was mutated into a stop codon. AGA to TGA. Dominant negative mutant.

name

DPC4 (1-514)

shRNA_sequence

size

1600

species

9606

Homo sapiens