RCAS(A) mdnTcf4Ben (CT#1130) | Addgene 14020 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

RCAS(A) mdnTcf4Ben (CT#1130)

catalog :

14020

citations: 1

Reference
Kardon G, Harfe B, Tabin C. A Tcf4-positive mesodermal population provides a prepattern for vertebrate limb muscle patterning. Dev Cell. 2003;5:937-44 pubmed

product information

Catalog Number :

14020

Product Name :

RCAS(A) mdnTcf4Ben (CT#1130)

article :

doi	10.1016/S1534-5807(03)00360-5
id	1217
pubmed_id	14667415

bacterial resistance :

Ampicillin

cloning :

backbone

RCASBP(A)

backbone_mutation

backbone_origin

backbone_size

11600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Retroviral

Other

avian expression

growth notes :

RCAS dnTcf4en virus was constructed using the mouse Tcf4B splice variant (gift of G. Dressler), as the chick Tcf4B splice variant is expressed in the developing limbs. The dominant-negative (dn) form was created by deleting amino acids 2 50, resulting in the production of a putative dominant-negative protein unable to bind and be activated by beta-catenin, but able to bind DNA via its C-terminal HMG box. The repressor domain from the Drosophila engrailed gene was fused in-frame to the 3 end of the dnTcf4 construct in the shuttle vector pSlax13 and then subcloned into retroviral vector RCAS(BP) A.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	ClaI
cloning_site_5	ClaI
promoter
sequencing_primer_3
sequencing_primer_5	RCAS-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	TCF4B, TCF4E, Tcf-4, Tcf4
gene	Tcf7l2
id	21416

genbank_ids

mutation

Dominant negative Tcf4B, engrailed fusion

name

Tcf4B

shRNA_sequence

size

species

10090

Mus musculus