pcDNA-GalNAc-T2-GFP-ESCargo(FTV) | Addgene 140163 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

catalog :

140163

citations: 2

Reference
Casler J, Zajac A, Valbuena F, Sparvoli D, Jeyifous O, Turkewitz A, et al. ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms. Mol Biol Cell. 2020;31:2892-2903 pubmed publisher
Valbuena F, Fitzgerald I, Strack R, Andruska N, Smith L, Glick B. A photostable monomeric superfolder green fluorescent protein. Traffic. 2020;: pubmed publisher

product information

Catalog Number :

140163

Product Name :

pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

article :

doi	10.1091/mbc.E20-09-0591
id	28215304
pubmed_id	33112725

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA5/FRT/TO

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.

growth strain :

Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	EF-1a
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	GalNAc-T2
gene	GALNT2
id	2590

genbank_ids

mutation

name

GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)

shRNA_sequence

size

species

9606

Homo sapiens

100

Synthetic