This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RCAS (A) goosecoid (CT#19)
catalog :
13984
citations: 1
Reference
Heanue T, Johnson R, Izpisua Belmonte J, Stern C, De Robertis E, Tabin C. Goosecoid misexpression alters the morphology and Hox gene expression of the developing chick limb bud. Mech Dev. 1997;69:31-7 pubmed
product information
Catalog Number :
13984
Product Name :
RCAS (A) goosecoid (CT#19)
article :
doi10.1016/S0925-4773(97)00149-4
id1161
pubmed_id9486529
bacterial resistance :
Ampicillin
cloning :
backboneRCASBP(A)
backbone_mutation
backbone_origin
backbone_size11600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
Other
avian expression
growth notes :
gsc ORF ligated into NcoI-Sal I of the Cla 12 Nco adaptor and then cloned into the Cla I site of RCAS (A). Addgene QC NGS analysis identifies a P2A amino acid discrepancy in the goosecoid insert sequence, relative to NP_990662.2. It is unknown what, if any, impact this may have on plasmid function.
origin :
37
pi :
alt_names
gsc
cloning
clone_methodRestriction Enzyme
cloning_site_3ClaI
cloning_site_5ClaI
promoter
sequencing_primer_3
sequencing_primer_5RCAS-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneGSC
id396273
genbank_ids
mutation
namegoosecoid
shRNA_sequence
size700
species
9031
Gallus gallus
tags
plasmid copy :
Juan Carlos Ispisua Bel monte
resistance markers :
514
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA