This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
catalog :
139482
citations: 1
Reference
Uda Y, Miura H, Goto Y, Yamamoto K, Mii Y, Kondo Y, et al. Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics. ACS Chem Biol. 2020;15:2896-2906 pubmed publisher
product information
Catalog Number :
139482
Product Name :
pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
article :
doi10.1021/acschembio.0c00477
id28207674
pubmed_id33164485
bacterial resistance :
Ampicillin
cloning :
backbonepCSIIneo-TRE
backbone_mutation
backbone_origin
backbone_size9668
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Depositor confirmed that mutation G229L and deletion of amino acid Y at position 235 in mCherry has no functional effect.
growth strain :
Expresses PhyB621-mCherry-HRasCT in mammalian cells.
origin :
37
pi :
alt_names
phytochrome b
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterTet responsible element
sequencing_primer_3GGGCCCTCACATTGCCAAA
sequencing_primer_5not determined
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
genePHYB
id816394
genbank_ids
mutationG229L and deletion of aa Y at position 235 in mcherry
namePhyB621
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagmCherry-HRasCT
resistance markers :
3577
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA