RCAS (A) Flu cPitx2 (CT#704) | Addgene 13928 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

RCAS (A) Flu cPitx2 (CT#704)

catalog :

13928

citations: 1

Reference
Logan M, Pagán Westphal S, Smith D, Paganessi L, Tabin C. The transcription factor Pitx2 mediates situs-specific morphogenesis in response to left-right asymmetric signals. Cell. 1998;94:307-17 pubmed

product information

Catalog Number :

13928

Product Name :

RCAS (A) Flu cPitx2 (CT#704)

article :

doi	10.1016/S0092-8674(00)81474-9
id	1201
pubmed_id	9708733

bacterial resistance :

Ampicillin

cloning :

backbone

RCASBP(A)

backbone_mutation

backbone_origin

backbone_size

11600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Retroviral

Other

avian expression

growth notes :

The entire open reading frame of cPitx2 was generated by high fidelity PCR and cloned into a version of pSlax13 containing a triple HA (Flu) tag (Laufer et al., 1997). The sequence of the PCR-generated insert was confirmed by sequencing. A ClaI fragment containing the cPitx2-Flu sequence was cloned into the RCAS retroviral vector.

origin :

pi :

alt_names

Ptx2

cloning

clone_method	Restriction Enzyme
cloning_site_3	ClaI
cloning_site_5	ClaI
promoter
sequencing_primer_3
sequencing_primer_5	RCAS-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	PITX2
id	395862

genbank_ids

mutation

name

pitx2

shRNA_sequence

size

species

9031

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