pLenti-CMV-Blast-PIP-FUCCI | Addgene 138715 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti-CMV-Blast-PIP-FUCCI

catalog :

138715

citations: 3

Reference
Yedier Bayram O, Gokbayrak B, Kayabolen A, Aksu A, Cavga A, Cing xf6 z A, et al. EPIKOL, a chromatin-focused CRISPR/Cas9-based screening platform, to identify cancer-specific epigenetic vulnerabilities. Cell Death Dis. 2022;13:710 pubmed publisher
Sela Y, Li J, Maheswaran S, Norgard R, Yuan S, Hubbi M, et al. Bcl-xL Enforces a Slow-Cycling State Necessary for Survival in the Nutrient-Deprived Microenvironment of Pancreatic Cancer. Cancer Res. 2022;82:1890-1908 pubmed publisher
Grant G, Kedziora K, Limas J, Cook J, Purvis J. Accurate delineation of cell cycle phase transitions in living cells with PIP-FUCCI. Cell Cycle. 2018;17:2496-2516 pubmed publisher

product information

Catalog Number :

138715

Product Name :

pLenti-CMV-Blast-PIP-FUCCI

article :

doi	10.1080/15384101.2018.1547001
id	28196778
pubmed_id	30421640

bacterial resistance :

Ampicillin

cloning :

backbone

pLenti CMV Blast DEST (706 1)

backbone_mutation

backbone_origin

backbone_size

9331

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth strain :

Lentiviral expression of the cell cycle marker PIP-FUCCI

origin :

pi :

alt_names

PIP-NLS-mVenus-P2A-mCherry-Gem(1-110)

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV promoter primer (5'-ATG GGC GGT AGG CGT GTA CG)
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

PIP-FUCCI

shRNA_sequence

size

1992

species

100

Synthetic