This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pACDura3
catalog :
138711
citations: 1
Reference
Velázquez E, Lorenzo V, Al Ramahi Y. Recombination-Independent Genome Editing through CRISPR/Cas9-Enhanced TargeTron Delivery. ACS Synth Biol. 2019;8:2186-2193 pubmed publisher
product information
Catalog Number :
138711
Product Name :
pACDura3
article :
doi10.1021/acssynbio.9b00293
id28207595
pubmed_id31419111
bacterial resistance :
Chloramphenicol
cloning :
backbonepACD4K-C
backbone_mutationFirst, retro-transposition-activated selectable marker (RAM) in pACD4K-C was excised by digestion with mluI enzyme. Then, optimized ura3 gene was amplified by PCR, digested with mluI and ligated with mluI-digested pACD4K-C vector.
backbone_originSigma-Aldrich
backbone_size7675
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3mluI
cloning_site_5mluI
promoterpEM7 promoter
sequencing_primer_3ATGATAATATCAGAGCCGGTAG
sequencing_primer_5ATGTCCAAGGCTACTTACAAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYEL021W
geneURA3
id856692
genbank_ids
mutationoptimized ura3 gene for expression in Escherichia coli
nameura3 gene
shRNA_sequence
size963
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
2100
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA