pGEX Su(fu) (CT#99) | Addgene 13856 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGEX Su(fu) (CT#99)

catalog :

13856

citations: 1

Reference
Pearse R, Collier L, Scott M, Tabin C. Vertebrate homologs of Drosophila suppressor of fused interact with the gli family of transcriptional regulators. Dev Biol. 1999;212:323-36 pubmed

product information

Catalog Number :

13856

Product Name :

pGEX Su(fu) (CT#99)

article :

doi	10.1006/dbio.1999.9335
id	1191
pubmed_id	10433824

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX-KG

backbone_mutation

backbone_origin

ATCC

backbone_size

5000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The GST Su(fu) construct was generated by using the bacterial expression vector pGEX-KG to fuse glutathione S-transferase in-frame with a 2.0-kb NcoI chick Su(fu) fragment extending from the initial methionine (the ATG in the 5' NcoI site) to 600 bp past the stop codon.

origin :

pi :

alt_names

suppressor of fused

cloning

clone_method	Restriction Enzyme
cloning_site_3	NcoI
cloning_site_5	NcoI
promoter
sequencing_primer_3	pGEX 3'
sequencing_primer_5	pGEX 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases
gene	SUFU
id	374118

genbank_ids

mutation

Nco I fragment from full length; ~2kbp- ATG to stop plus 3 UTR.

name

Su(fu)

shRNA_sequence

size

2000

species

9031

Gallus gallus