This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pYES2/103Q
catalog :
1385
citations: 3
Reference
Williams F, Wu Y, Scaglione K. Development of a Positive Selection High Throughput Genetic Screen in Dictyostelium discoideum. Front Cell Dev Biol. 2021;9:725678 pubmed publisher
Zilio N, Boddy M. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2017;2017: pubmed publisher
Meriin A, Zhang X, He X, Newnam G, Chernoff Y, Sherman M. Huntington toxicity in yeast model depends on polyglutamine aggregation mediated by a prion-like protein Rnq1. J Cell Biol. 2002;157:997-1004 pubmed
product information
Catalog Number :
1385
Product Name :
pYES2/103Q
article :
doi10.1083/jcb.200112104
id10
pubmed_id12058016
bacterial resistance :
Ampicillin
cloning :
backbonepYES2
backbone_mutation
backbone_originInvitrogen
backbone_size5900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
URA3 selection, 2micron origin
origin :
37
pi :
alt_names
htt
huntingtin
HD
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoter
sequencing_primer_3
sequencing_primer_5GAL1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHD, IT15, LOMARS
geneHTT
id3064
genbank_ids
AB016794
mutationExon1 sequences containing the first 17 amino acids followed by 103 glutamines were fused in frame
namehuntingtin (103Q)
shRNA_sequence
size1800
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
locationC terminal on insert
tagEGFP
resistance markers :
468
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA