This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCiB-yin
catalog :
13845
citations: 1
Reference
Bateman J, Lee A, Wu C. Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Genetics. 2006;173:769-77 pubmed
product information
Catalog Number :
13845
Product Name :
pCiB-yin
article :
doi10.1534/genetics.106.056945
id1185
pubmed_id16547094
bacterial resistance :
Ampicillin
cloning :
backbonepCar4
backbone_mutation
backbone_origin
backbone_size2500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
pCiB-yin is a donor vector that contains an intronless yellow gene flanked by inverted attB sites. The cassette was constructed in the parent vector pCar4. In terms of use, there are no convenient restriction sites available to use pCiB-yin as a starting point for cloning (piB-GFP is more useful for that), but pCiB-yin provides a useful positive control for injections since insertion of the cassette produces a dominant pigmentation phenotype in a yellow background.
origin :
37
pi :
alt_names
y
cloning
clone_methodRestriction Enzyme
cloning_site_3attB
cloning_site_5attB
promoter
sequencing_primer_3
sequencing_primer_5Pry1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG3757, CG3757, Dmel CG3757, EG:125H10.2, T6, Y
geney
id30980
genbank_ids
mutation
nameyellow
shRNA_sequence
size7000
species
7227
Drosophila melanogaster
tags
plasmid copy :
attB from Michele Calos, Stanford
resistance markers :
590
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA