This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUASTP2.1
catalog :
13842
citations: 1
Reference
Bateman J, Lee A, Wu C. Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Genetics. 2006;173:769-77 pubmed
product information
Catalog Number :
13842
Product Name :
pUASTP2.1
article :
doi10.1534/genetics.106.056945
id1185
pubmed_id16547094
bacterial resistance :
Ampicillin
cloning :
backbonepUAST
backbone_mutation
backbone_origin
backbone_size4300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
pUASTP2.1 contains a P-element target for RMCE, the mini-white gene flanked by inverted attP sites. It is a derivative of PUASTP2 in which the UAS and TATA sequences flanking the cassette have been removed. (Specifically, PUASTP2 was digested with BamHI and StuI to remove UAS-TATA-attP, and an attP-containing BamHI-EcoRV fragment from pTA-attP was inserted). This vector can be used to generate target lines by standard P element transgenesis.
origin :
37
pi :
alt_names
white
cloning
clone_methodGateway Cloning
cloning_site_3attP
cloning_site_5attP
promoter
sequencing_primer_3
sequencing_primer_5Pry1
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG2759, BACN33B1.1, CG2759, DMWHITE, DmWhite, Dmel CG2759, EG:BACN33B1.1, W, White, c23, e(g), m(g), mini-white, mw, w(AT)[[13]]
genew
id31271
genbank_ids
mutation
namemini-white
shRNA_sequence
size4000
species
7227
Drosophila melanogaster
tags
plasmid copy :
attP from Michele Calos, Stanford
resistance markers :
590
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA