pMXs-EGFP-Rheb-HVR HRas-IP | Addgene 13833 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMXs-EGFP-Rheb-HVR HRas-IP

catalog :

13833

citations: 1

Reference
Takahashi K, Nakagawa M, Young S, Yamanaka S. Differential membrane localization of ERas and Rheb, two Ras-related proteins involved in the phosphatidylinositol 3-kinase/mTOR pathway. J Biol Chem. 2005;280:32768-74 pubmed

product information

Catalog Number :

13833

Product Name :

pMXs-EGFP-Rheb-HVR HRas-IP

article :

doi	10.1074/jbc.M506280200
id	1181
pubmed_id	16046393

bacterial resistance :

Ampicillin

cloning :

backbone

pMX-EGFP-gw-IP

backbone_mutation

backbone_origin

backbone_size

6900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

origin :

pi :

alt_names

Rheb

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	EGFP-C
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	Rheb1
gene	Rheb
id	19744

genbank_ids

mutation

N-terminal of Rheb and hyper variable region (HVR) of HRas are fused in frame

name

Rheb-HVR HRas

shRNA_sequence

size

700

species

10090

Mus musculus

tags

location	N terminal on backbone
tag	EGFP

plasmid copy :

pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.

resistance markers :

596

tags :

High Copy

terms :

Puromycin