This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21
catalog :
138295
citations: 1
Reference
Park J, Burckhardt C, Lazcano R, Solis L, Isogai T, Li L, et al. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature. 2020;578:621-626 pubmed publisher
product information
Catalog Number :
138295
Product Name :
pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21
article :
doi10.1038/s41586-020-1998-1
id28196536
pubmed_id32051585
bacterial resistance :
Ampicillin
cloning :
backbonepSpCas9 wt (pSpCAS9(BB)-2A-GFP)
backbone_mutation
backbone_originFeng Zhang (Addgene plasmid # 48138)
backbone_size9300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth strain :
gRNA for CRISPR/Cas9 knockout of human TRIM21
origin :
37
pi :
alt_names
E3 ubiquitin-protein ligase TRIM21
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterU6
sequencing_primer_3
sequencing_primer_5U6 promoter forward: GACTATCATATGCTTACCGT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRNF81, RO52, Ro/SSA, SSA, SSA1
geneTRIM21
id6737
genbank_ids
mutation
namegRNA TRIM21
shRNA_sequenceGAAACACCGTGACCACGCCA
size20
species
9606
Homo sapiens
tags
resistance markers :
4142
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA