This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLVX neo PFKP-GFP K281R
catalog :
138288
citations: 1
Reference
Park J, Burckhardt C, Lazcano R, Solis L, Isogai T, Li L, et al. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature. 2020;578:621-626 pubmed publisher
product information
Catalog Number :
138288
Product Name :
pLVX neo PFKP-GFP K281R
article :
doi10.1038/s41586-020-1998-1
id28196536
pubmed_id32051585
bacterial resistance :
Ampicillin
cloning :
backbonepLVX neo
backbone_mutation
backbone_originClontech
backbone_size8140
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Mammalian Expression, Lentiviral. Expression of human phosphofructokinase platelet isoform PFKP-GFP fusion protein with K281R mutation
origin :
37
pi :
alt_names
ATP-dependent 6-phosphofructokinase, platelet type
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3IRES-R: CCTCACATTGCCAAAAGACG
sequencing_primer_5CMV forward: CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATP-PFK, PFK-C, PFK-P, PFKF
genePFKP
id5214
genbank_ids
mutationK281R
namePFKP
shRNA_sequence
size2355
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGFP
plasmid copy :
Thermo Ultimate lite Orfeome library: IOH4086; pENTR(tm)221
resistance markers :
4142
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA