cDNA

pFP.E222

138248

pFP.E222

Kanamycin

Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

37

Cloningcloning_site_3cloning_site_5promoteriJ23101sequencing_primer_3sequencing_primer_5(P768)_SspGyrB.ECF16.F - TTTGTCCATAACAGTGCAAAATTTGCAGCAAGTGATGAACAGCsite_3_destroyedsite_5_destroyedentrez_genegenbank_ids

NP_418341.1

mutationnamerhaSshRNA_sequencesize837species

32644 Other

Escherichia coli str. K-12 substr. MG1655

tags

alt_names
cloning	clone_method Gibson Cloning cloning_site_3 cloning_site_5 promoter P(rhaBAD) sequencing_primer_3 sequencing_primer_5 rhaS_F - GGATACTGCCCATCCAGCTC site_3_destroyed site_5_destroyed
entrez_gene
genbank_ids
mutation
name	ECF17.N.(NPC).NrdJ-1.N2
shRNA_sequence
size	609
species	100 Synthetic
tags

alt_names	ECK120033737
cloning
entrez_gene
genbank_ids
mutation
name	Terminator
shRNA_sequence
size	57
species	100 Synthetic
tags

alt_names
cloning	clone_method Gibson Cloning cloning_site_3 cloning_site_5 promoter P(rhaBAD) sequencing_primer_3 sequencing_primer_5 (P769)_NrdJ-1.N.FII - AATCCGTGTTGTCTGGTTGGTAGCAGC site_3_destroyed site_5_destroyed
entrez_gene
genbank_ids
mutation
name	M86.C2.(SDL).ECF20.C
shRNA_sequence
size	435
species	100 Synthetic
tags

alt_names	B0015
cloning
entrez_gene
genbank_ids
mutation
name	Terminator
shRNA_sequence
size	129
species
tags

alt_names	VFA0925
cloning	clone_method Gibson Cloning cloning_site_3 cloning_site_5 promoter J23115 sequencing_primer_3 sequencing_primer_5 (P493)_E20C.F - ACCCGTCCGGCACCGGATGAACAGCTGGAAGC site_3_destroyed site_5_destroyed
entrez_gene
genbank_ids	YP_206883.1
mutation
name	luxR
shRNA_sequence
size	753
species	32644 Other Vibrio fischeri ES114
tags

alt_names	B0015
cloning
entrez_gene
genbank_ids
mutation
name	Terminator
shRNA_sequence
size	129
species	100 Synthetic
tags

alt_names
cloning	clone_method Gibson Cloning cloning_site_3 cloning_site_5 promoter P(lux2) sequencing_primer_3 sequencing_primer_5 LuxR_seq.F - CAGTGAGCGTACTGTCACTTTCC site_3_destroyed site_5_destroyed
entrez_gene
genbank_ids
mutation
name	ECF16.N.(AGG).SspGyrB.N2
shRNA_sequence
size	684
species	100 Synthetic
tags

alt_names	L3S2P21
cloning
entrez_gene
genbank_ids
mutation
name	Terminator
shRNA_sequence
size	61
species	100 Synthetic
tags

alt_names
cloning	clone_method Gibson Cloning cloning_site_3 cloning_site_5 promoter P(lux2) sequencing_primer_3 VR - ATTACCGCCTTTGAGTGAGC sequencing_primer_5 LuxR_seq.F - CAGTGAGCGTACTGTCACTTTCC site_3_destroyed site_5_destroyed
entrez_gene
genbank_ids
mutation
name	NrdJ-1.C2.(SEI).ECF17.C
shRNA_sequence
size	363
species	100 Synthetic
tags

AGCGAACATGCCTGTGAAGTGGATGCTTGCATCTCGGGA
GATAGTTTGATCAGCsite_3
_destroyedsi
te_5_destroyede>entrez_gene>genbank_ids
mutation>nameSspGyrB.C2.(
SAK).ECF16.CshRNA_seq
uencesized>378species
>tags

ble> 100td> Synthetic

tr>

alt_names>	ble>tr> r>able> B0015 cloning < /td> entrez_gene td> genbank_ids d> mutation name Terminator shRNA_sequence d> size 129 species d> 100 S ynthetic tags d>
>
alt_names	< td>b3905< /tr> ECK3898 rhaC2 >
cloning	r> clone_method Gibson alt_names cloning clone_method Gibson Cloning cloning_site_3 cloning_site_5 promoter iPC sequencing_primer_3 sequencing_primer_5 VF2 - TGCCACCTGACGTCTAAGAA site_3_destroyed site_5_destroyed entrez_gene aliases b0064, ECK0065 gene araC id 944780 genbank_ids mutation name araC shRNA_sequence size 930 species 32644 Other tags alt_names cloning clone_method Gibson Cloning cloning_site_3 cloning_site_5 promoter P(araBAD) sequencing_primer_3 sequencing_primer_5 araC_F2 - AACCCACTGGTGATACCATTCG site_3_destroyed site_5_destroyed entrez_gene genbank_ids mutation name ECF20.N.(VDA).M86.N2 shRNA_sequence size 624 species 100 Synthetic tags alt_names L3S2P21 cloning entrez_gene genbank_ids mutation name Terminator shRNA_sequence size 61 species 100 Synthetic tags alt_names cloning clone_method Gibson Cloning cloning_site_3 cloning_site_5 promoter P(araBAD) sequencing_primer_3 sequencing_primer_5 (P484)_E20N.M86.F - resistance markers : 2181 tags : Low Copy company information Addgene 490 Arsenal Way, Suite 100 Watertown, MA 02472 info@addgene.org https://www.addgene.org 617.225.9000 headquarters: USA questions and comments   Labome.com © 2024 All Rights Reserved last updated:2025-07-08