This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFP.R369
catalog :
138239
citations: 1
Reference
Pinto F, Thornton E, Wang B. An expanded library of orthogonal split inteins enables modular multi-peptide assemblies. Nat Commun. 2020;11:1529 pubmed publisher
product information
Catalog Number :
138239
Product Name :
pFP.R369
article :
doi10.1038/s41467-020-15272-2
id28207507
pubmed_id32251274
bacterial resistance :
Tetracycline
cloning :
backbonepSB3T5
backbone_mutation
backbone_originBioBrick vector, IGEM registry
backbone_size3252
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Synthetic Biology
growth strain :
Expresses the split mCherry reporter interrupted by the MP-K-gp53 intein (in cis)
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoteriPC
sequencing_primer_3
sequencing_primer_5VF2 - TGCCACCTGACGTCTAAGAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0064, ECK0065
genearaC
id944780
genbank_ids
mutation
namearaC
shRNA_sequence
size930
species
32644
Other
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterP(araBAD)
sequencing_primer_3VR - ATTACCGCCTTTGAGTGAGC
sequencing_primer_5araC_F2 - AACCCACTGGTGATACCATTCG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namemCherryN(NHD).MP-K-gp53*.(SRA)mCherryC.H6
shRNA_sequence
size1197
species
100
Synthetic
tags
locationC terminal on insert
tag6xHis
resistance markers :
2181
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA