This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SIRT7 Flag
catalog :
13818
citations: 8
Reference
Vigili de Kreutzenberg S, Giannella A, Ceolotto G, Faggin E, Cappellari R, Mazzucato M, et al. A miR-125/Sirtuin-7 pathway drives the pro-calcific potential of myeloid cells in diabetic vascular disease. Diabetologia. 2022;: pubmed publisher
Garcia Morato J, Hans F, von Zweydorf F, Feederle R, Els xe4 sser S, Skodras A, et al. Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43. Nat Commun. 2022;13:1223 pubmed publisher
Shimizu K, Gi M, Suzuki S, North B, Watahiki A, Fukumoto S, et al. Interplay between protein acetylation and ubiquitination controls MCL1 protein stability. Cell Rep. 2021;37:109988 pubmed publisher
Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
Mohibi S, Srivastava S, Bele A, Mirza S, Band H, Band V. Acetylation of Mammalian ADA3 Is Required for Its Functional Roles in Histone Acetylation and Cell Proliferation. Mol Cell Biol. 2016;36:2487-502 pubmed publisher
Ronnebaum S, Wu Y, McDonough H, Patterson C. The ubiquitin ligase CHIP prevents SirT6 degradation through noncanonical ubiquitination. Mol Cell Biol. 2013;33:4461-72 pubmed publisher
Fatoba S, Tognetti S, Berto M, Leo E, Mulvey C, Godovac Zimmermann J, et al. Human SIRT1 regulates DNA binding and stability of the Mcm10 DNA replication factor via deacetylation. Nucleic Acids Res. 2013;41:4065-79 pubmed publisher
North B, Marshall B, Borra M, Denu J, Verdin E. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol Cell. 2003;11:437-44 pubmed
product information
Catalog Number :
13818
Product Name :
SIRT7 Flag
article :
doi10.1016/S1097-2765(03)00038-8
id1178
pubmed_id12620231
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 +
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details). Missing exon 2: nucleotides 127-264 of NM_016538.
growth strain :
Mammalian expression of human SIRT7 with flag tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3BGH Reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSIR2L7
geneSIRT7
id51547
genbank_ids
mutationMissing exon 2
nameSIRT7
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagflag
plasmid copy :
Roy Frye
resistance markers :
547
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA