This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SIRT3 Flag
catalog :
13814
citations: 23
Reference
Tiwari A, Hashemiaghdam A, Laramie M, Maschi D, Haddad T, Stunault M, et al. Sirtuin3 ensures the metabolic plasticity of neurotransmission during glucose deprivation. bioRxiv. 2023;: pubmed publisher
Masgras I, Cannino G, Ciscato F, Sanchez Martin C, Darvishi F, Scantamburlo F, et al. Tumor growth of neurofibromin-deficient cells is driven by decreased respiration and hampered by NAD+ and SIRT3. Cell Death Differ. 2022;: pubmed publisher
Osborne B, Reznick J, Wright L, Sinclair D, Cooney G, Turner N. Liver-specific overexpression of SIRT3 enhances oxidative metabolism, but does not impact metabolic defects induced by high fat feeding in mice. Biochem Biophys Res Commun. 2022;607:131-137 pubmed publisher
Garcia Morato J, Hans F, von Zweydorf F, Feederle R, Els xe4 sser S, Skodras A, et al. Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43. Nat Commun. 2022;13:1223 pubmed publisher
Shimizu K, Gi M, Suzuki S, North B, Watahiki A, Fukumoto S, et al. Interplay between protein acetylation and ubiquitination controls MCL1 protein stability. Cell Rep. 2021;37:109988 pubmed publisher
Lambhate S, Bhattacharjee D, Jain N. APC/C CDH1 ubiquitinates IDH2 contributing to ROS increase in mitosis. Cell Signal. 2021;86:110087 pubmed publisher
Gan L, Liu D, Liu J, Chen E, Chen C, Liu L, et al. CD38 deficiency alleviates Ang II-induced vascular remodeling by inhibiting small extracellular vesicle-mediated vascular smooth muscle cell senescence in mice. Signal Transduct Target Ther. 2021;6:223 pubmed publisher
Sawant Dessai A, Domínguez M, Chen U, Hasper J, Prechtl C, Yu C, et al. Transcriptional Repression of SIRT3 Potentiates Mitochondrial Aconitase Activation to Drive Aggressive Prostate Cancer to the Bone. Cancer Res. 2021;81:50-63 pubmed publisher
Chi Z, Le T, Lee S, Guo E, Kim D, Lee S, et al. Honokiol ameliorates angiotensin II-induced hypertension and endothelial dysfunction by inhibiting HDAC6-mediated cystathionine γ-lyase degradation. J Cell Mol Med. 2020;: pubmed publisher
Podgorski I, Pinterić M, Marčinko D, Popović Hadzija M, Filic V, Ciganek I, et al. Combination of sirtuin 3 and hyperoxia diminishes tumorigenic properties of MDA-MB-231 cells. Life Sci. 2020;254:117812 pubmed publisher
Maniyadath B, Chattopadhyay T, Verma S, Kumari S, Kulkarni P, Banerjee K, et al. Loss of Hepatic Oscillatory Fed microRNAs Abrogates Refed Transition and Causes Liver Dysfunctions. Cell Rep. 2019;26:2212-2226.e7 pubmed publisher
Si Y, Bao H, Han L, Chen L, Zeng L, Jing L, et al. Dexmedetomidine attenuation of renal ischaemia-reperfusion injury requires sirtuin 3 activation. Br J Anaesth. 2018;121:1260-1271 pubmed publisher
Chhipa R, Fan Q, Anderson J, Muraleedharan R, Huang Y, Ciraolo G, et al. AMP kinase promotes glioblastoma bioenergetics and tumour growth. Nat Cell Biol. 2018;20:823-835 pubmed publisher
Pinterić M, Podgorski I, Sobocanec S, Popović Hadzija M, Paradzik M, Dekanic A, et al. De novo expression of transfected sirtuin 3 enhances susceptibility of human MCF-7 breast cancer cells to hyperoxia treatment. Free Radic Res. 2018;52:672-684 pubmed publisher
Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
Li R, Quan Y, Xia W. SIRT3 inhibits prostate cancer metastasis through regulation of FOXO3A by suppressing Wnt/β-catenin pathway. Exp Cell Res. 2018;364:143-151 pubmed publisher
Lee D, Jung D, Yu S, Lee Y, Choi B, Lee Y. Regulation of SIRT3 signal related metabolic reprogramming in gastric cancer by Helicobacter pylori oncoprotein CagA. Oncotarget. 2017;8:78365-78378 pubmed publisher
Chen M, Zhu X, Ran L, Lang H, Yi L, Mi M. Trimethylamine-N-Oxide Induces Vascular Inflammation by Activating the NLRP3 Inflammasome Through the SIRT3-SOD2-mtROS Signaling Pathway. J Am Heart Assoc. 2017;6: pubmed publisher
Quan Y, Wang N, Chen Q, Xu J, Cheng W, Di M, et al. SIRT3 inhibits prostate cancer by destabilizing oncoprotein c-MYC through regulation of the PI3K/Akt pathway. Oncotarget. 2015;6:26494-507 pubmed publisher
Xu Z, Zhang L, Zhang W, Meng D, Zhang H, Jiang Y, et al. SIRT6 rescues the age related decline in base excision repair in a PARP1-dependent manner. Cell Cycle. 2015;14:269-76 pubmed publisher
Song Y, Shi J, Wu Y, Han C, Zou J, Shi Y, et al. Metformin ameliorates insulin resistance in L6 rat skeletal muscle cells through upregulation of SIRT3. Chin Med J (Engl). 2014;127:1523-9 pubmed
Buler M, Aatsinki S, Izzi V, Hakkola J. Metformin reduces hepatic expression of SIRT3, the mitochondrial deacetylase controlling energy metabolism. PLoS ONE. 2012;7:e49863 pubmed publisher
North B, Marshall B, Borra M, Denu J, Verdin E. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol Cell. 2003;11:437-44 pubmed
product information
Catalog Number :
13814
Product Name :
SIRT3 Flag
article :
doi10.1016/S1097-2765(03)00038-8
id1178
pubmed_id12620231
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 +
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).
growth strain :
Mammalian expression of human SIRT3 with flag tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3BGH Reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSIR2L3
geneSIRT3
id23410
genbank_ids
mutation
nameSIRT3
shRNA_sequence
size1199
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagflag
plasmid copy :
Roy Frye
resistance markers :
547
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA