This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SIRT2 Flag
catalog :
13813
citations: 22
Reference
Villar Pazos S, Thomas L, Yang Y, Chen K, Lyles J, Deitch B, et al. Neural deficits in a mouse model of PACS1 syndrome are corrected with PACS1- or HDAC6-targeting therapy. Nat Commun. 2023;14:6547 pubmed publisher
Thomas G, Villar Pazos S, Thomas L, Yang Y, Chen K, Lyles J, et al. RNA-targeted therapy corrects neuronal deficits in PACS1 syndrome mice. Res Sq. 2023;: pubmed publisher
Garcia Morato J, Hans F, von Zweydorf F, Feederle R, Els xe4 sser S, Skodras A, et al. Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43. Nat Commun. 2022;13:1223 pubmed publisher
Shimizu K, Gi M, Suzuki S, North B, Watahiki A, Fukumoto S, et al. Interplay between protein acetylation and ubiquitination controls MCL1 protein stability. Cell Rep. 2021;37:109988 pubmed publisher
Curry A, Cohen I, Zheng S, Wohlfahrt J, White D, Donu D, et al. Profiling sirtuin activity using Copper-free click chemistry. Bioorg Chem. 2021;117:105413 pubmed publisher
Minten E, Kapoor Vazirani P, Li C, Zhang H, Balakrishnan K, Yu D. SIRT2 promotes BRCA1-BARD1 heterodimerization through deacetylation. Cell Rep. 2021;34:108921 pubmed publisher
Hisahara S, Iwahara N, Matsushita T, Suzuki S, Matsumura A, Fujikura M, et al. SIRT1 decelerates morphological processing of oligodendrocyte cell lines and regulates the expression of cytoskeleton-related oligodendrocyte proteins. Biochem Biophys Res Commun. 2021;546:7-14 pubmed publisher
Zheng S, Wohlfahrt J, Cohen I, Cen Y. Methods for studying human sirtuins with activity-based chemical probes. Methods Enzymol. 2020;633:251-269 pubmed publisher
Wang X, Buechler N, Long D, Furdui C, Yoza B, McCall C, et al. Cysteine thiol oxidation on SIRT2 regulates inflammation in obese mice with sepsis. Inflammation. 2019;42:156-169 pubmed publisher
Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
Graham E, Rymarchyk S, Wood M, Cen Y. Development of Activity-Based Chemical Probes for Human Sirtuins. ACS Chem Biol. 2018;13:782-792 pubmed publisher
Fischer A, Mühlhäuser W, Warscheid B, Radziwill G. Membrane localization of acetylated CNK1 mediates a positive feedback on RAF/ERK signaling. Sci Adv. 2017;3:e1700475 pubmed publisher
Singh P, Hanson P, Morris C. Sirtuin-2 Protects Neural Cells from Oxidative Stress and Is Elevated in Neurodegeneration. Parkinsons Dis. 2017;2017:2643587 pubmed publisher
Head P, Zhang H, Bastien A, Koyen A, Withers A, Daddacha W, et al. Sirtuin 2 mutations in human cancers impair its function in genome maintenance. J Biol Chem. 2017;292:9919-9931 pubmed publisher
Wang X, Buechler N, Martin A, Wells J, Yoza B, McCall C, et al. Sirtuin-2 Regulates Sepsis Inflammation in ob/ob Mice. PLoS ONE. 2016;11:e0160431 pubmed publisher
Liu H, Jiang F, Loo Y, Hsu S, Hsiang T, Marcotrigiano J, et al. Regulation of Retinoic Acid Inducible Gene-I (RIG-I) Activation by the Histone Deacetylase 6. EBioMedicine. 2016;9:195-206 pubmed publisher
Liu L, Arun A, Ellis L, Peritore C, Donmez G. SIRT2 enhances 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced nigrostriatal damage via apoptotic pathway. Front Aging Neurosci. 2014;6:184 pubmed publisher
Li Z, Xie Q, Chen Z, Lu S, Xia W. Regulation of SIRT2 levels for human non-small cell lung cancer therapy. Lung Cancer. 2013;82:9-15 pubmed publisher
Sunami Y, Araki M, Hironaka Y, Morishita S, Kobayashi M, Liew E, et al. Inhibition of the NAD-dependent protein deacetylase SIRT2 induces granulocytic differentiation in human leukemia cells. PLoS ONE. 2013;8:e57633 pubmed publisher
Fatoba S, Tognetti S, Berto M, Leo E, Mulvey C, Godovac Zimmermann J, et al. Human SIRT1 regulates DNA binding and stability of the Mcm10 DNA replication factor via deacetylation. Nucleic Acids Res. 2013;41:4065-79 pubmed publisher
Snider N, Leonard J, Kwan R, Griggs N, Rui L, Omary M. Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation. J Cell Biol. 2013;200:241-7 pubmed publisher
North B, Marshall B, Borra M, Denu J, Verdin E. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol Cell. 2003;11:437-44 pubmed
product information
Catalog Number :
13813
Product Name :
SIRT2 Flag
article :
doi10.1016/S1097-2765(03)00038-8
id1178
pubmed_id12620231
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 +
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details). This variant (2) lacks the additional 47 nt present in the 5' end of the coding region in transcript variant 1. Translation is thought to initiate at a downstream in-frame ATG.
growth strain :
Mammalian expression of human SIRT2 with flag tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3BGH Reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSIR2, SIR2L, SIR2L2
geneSIRT2
id22933
genbank_ids
mutationTranscript variant 2
nameSIRT2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagflag
plasmid copy :
Roy Frye
resistance markers :
547
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA