This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
SIRT1 Flag
catalog :
13812
citations: 15
Reference
Garcia Morato J, Hans F, von Zweydorf F, Feederle R, Els xe4 sser S, Skodras A, et al. Sirtuin-1 sensitive lysine-136 acetylation drives phase separation and pathological aggregation of TDP-43. Nat Commun. 2022;13:1223 pubmed publisher
Shimizu K, Gi M, Suzuki S, North B, Watahiki A, Fukumoto S, et al. Interplay between protein acetylation and ubiquitination controls MCL1 protein stability. Cell Rep. 2021;37:109988 pubmed publisher
Gan L, Liu D, Liu J, Chen E, Chen C, Liu L, et al. CD38 deficiency alleviates Ang II-induced vascular remodeling by inhibiting small extracellular vesicle-mediated vascular smooth muscle cell senescence in mice. Signal Transduct Target Ther. 2021;6:223 pubmed publisher
Höllmüller E, Geigges S, Niedermeier M, Kammer K, Kienle S, Rösner D, et al. Site-specific ubiquitylation acts as a regulator of linker histone H1. Nat Commun. 2021;12:3497 pubmed publisher
Lei B, Wang K, Yang D, Liao L, Dong X, Huang Z. Co-culture with Sirt1-overexpressed chondrocytes delays the nucleus pulposus cells degeneration. Cell Tissue Bank. 2021;: pubmed publisher
Hisahara S, Iwahara N, Matsushita T, Suzuki S, Matsumura A, Fujikura M, et al. SIRT1 decelerates morphological processing of oligodendrocyte cell lines and regulates the expression of cytoskeleton-related oligodendrocyte proteins. Biochem Biophys Res Commun. 2021;546:7-14 pubmed publisher
Chen X, Huan H, Liu C, Luo Y, Shen J, Zhuo Y, et al. Deacetylation of β-catenin by SIRT1 regulates self-renewal and oncogenesis of liver cancer stem cells. Cancer Lett. 2019;463:1-10 pubmed publisher
Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
Liu H, Jiang F, Loo Y, Hsu S, Hsiang T, Marcotrigiano J, et al. Regulation of Retinoic Acid Inducible Gene-I (RIG-I) Activation by the Histone Deacetylase 6. EBioMedicine. 2016;9:195-206 pubmed publisher
Harlan B, Pehar M, Sharma D, Beeson G, Beeson C, Vargas M. Enhancing NAD+ Salvage Pathway Reverts the Toxicity of Primary Astrocytes Expressing Amyotrophic Lateral Sclerosis-linked Mutant Superoxide Dismutase 1 (SOD1). J Biol Chem. 2016;291:10836-46 pubmed publisher
Thompson A, Martin K, Rzucidlo E. Resveratrol induces vascular smooth muscle cell differentiation through stimulation of SirT1 and AMPK. PLoS ONE. 2014;9:e85495 pubmed publisher
Shin H, Decotiis J, Giron M, Palmeri D, Lukac D. Histone deacetylase classes I and II regulate Kaposi's sarcoma-associated herpesvirus reactivation. J Virol. 2014;88:1281-92 pubmed publisher
Ronnebaum S, Wu Y, McDonough H, Patterson C. The ubiquitin ligase CHIP prevents SirT6 degradation through noncanonical ubiquitination. Mol Cell Biol. 2013;33:4461-72 pubmed publisher
Madabushi A, Hwang B, Jin J, Lu A. Histone deacetylase SIRT1 modulates and deacetylates DNA base excision repair enzyme thymine DNA glycosylase. Biochem J. 2013;456:89-98 pubmed publisher
North B, Marshall B, Borra M, Denu J, Verdin E. The human Sir2 ortholog, SIRT2, is an NAD+-dependent tubulin deacetylase. Mol Cell. 2003;11:437-44 pubmed
product information
Catalog Number :
13812
Product Name :
SIRT1 Flag
article :
doi10.1016/S1097-2765(03)00038-8
id1178
pubmed_id12620231
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 +
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).
growth strain :
Mammalian expression of human SIRT1 with flag tag
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NotI
promoter
sequencing_primer_3BGH Reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSIR2, SIR2L1, SIR2alpha
geneSIRT1
id23411
genbank_ids
mutationN-terminus truncated
nameSIRT1
shRNA_sequence
size2000
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagflag
plasmid copy :
Roy Frye
resistance markers :
547
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA