This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pPN435
catalog :
137867
citations: 1
Reference
Hazelbaker D, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D, et al. A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells. Sci Rep. 2020;10:635 pubmed publisher
product information
Catalog Number :
137867
Product Name :
pPN435
article :
doi10.1038/s41598-020-57500-1
id28203657
pubmed_id31959800
bacterial resistance :
Spectinomycin
cloning :
backbonepX330S-3
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
Expression of gRNA i3 targeting TCF4 for CRISPRi; U6 promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5Unknown
promoterU6
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDG2T, E2-2, FCD2, FECD3, ITF-2, ITF2, PTHS, SEF-2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, SEF2-1D, TCF-4, bHLHb19
geneTCF4
id6925
genbank_ids
mutation
nameTCF4
shRNA_sequenceTTTCCTCAAACAATTCTTGT
size
species
9606
Homo sapiens
tags
resistance markers :
3391
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA