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product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-Cre
catalog :
13775
citations: 85
Reference
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Fan C, Kim D, An H, Park Y. Identifying an oligodendrocyte enhancer that regulates Olig2 expression. Hum Mol Genet. 2022;: pubmed publisher
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Nørgård M, Steffensen L, Hansen D, Füchtbauer E, Engelund M, Dimke H, et al. A new transgene mouse model using an extravesicular EGFP tag enables affinity isolation of cell-specific extracellular vesicles. Sci Rep. 2022;12:496 pubmed publisher
Geiller T, Sadeh S, Rolotti S, Blockus H, Vancura B, Negrean A, et al. Local circuit amplification of spatial selectivity in the hippocampus. Nature. 2022;601:105-109 pubmed publisher
Zhang L, Getz S, Bordey A. Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex. Front Bioeng Biotechnol. 2021;9:814638 pubmed publisher
Denoth Lippuner A, Jaeger B, Liang T, Royall L, Chie S, Buthey K, et al. Visualization of individual cell division history in complex tissues using iCOUNT. Cell Stem Cell. 2021;28:2020-2034.e12 pubmed publisher
Hsiao Y, Huang Y, Borah B, Chen S, Sun C. Single-laser-based simultaneous four-wavelength excitation source for femtosecond two-photon fluorescence microscopy. Biomed Opt Express. 2021;12:4661-4679 pubmed publisher
Mariani L, Guo X, Menezes N, Drozd A, Çakal S, Wang Q, et al. A TALE/HOX code unlocks WNT signalling response towards paraxial mesoderm. Nat Commun. 2021;12:5136 pubmed publisher
Panthi S, Schmitt P, Lorenz F, Stanfield B, Schmitt A. Paramyxovirus-Like Particles as Protein Delivery Vehicles. J Virol. 2021;95:e0103021 pubmed publisher
Kim D, An H, Fan C, Park Y. Identifying oligodendrocyte enhancers governing Plp1 expression. Hum Mol Genet. 2021;: pubmed publisher
Nagahama K, Fujino S, Watanabe T, Uesaka N, Kano M. Combining electrophysiology and optogenetics for functional screening of pyramidal neurons in the mouse prefrontal cortex. STAR Protoc. 2021;2:100469 pubmed publisher
van Heusden F, Macey Dare A, Gordon J, Krajeski R, Sharott A, Ellender T. Diversity in striatal synaptic circuits arises from distinct embryonic progenitor pools in the ventral telencephalon. Cell Rep. 2021;35:109041 pubmed publisher
Genolet O, Monaco A, Dunkel I, Boettcher M, Schulz E. Identification of X-chromosomal genes that drive sex differences in embryonic stem cells through a hierarchical CRISPR screening approach. Genome Biol. 2021;22:110 pubmed publisher
Benito Kwiecinski S, Giandomenico S, Sutcliffe M, Riis E, Freire Pritchett P, Kelava I, et al. An early cell shape transition drives evolutionary expansion of the human forebrain. Cell. 2021;184:2084-2102.e19 pubmed publisher
Brosh R, Laurent J, Ordoñez R, Huang E, Hogan M, Hitchcock A, et al. A versatile platform for locus-scale genome rewriting and verification. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Li X, Liu G, Yang L, Li Z, Zhang Z, Xu Z, et al. Decoding Cortical Glial Cell Development. Neurosci Bull. 2021;37:440-460 pubmed publisher
Mattar P, Jolicoeur C, Dang T, Shah S, Clark B, Cayouette M. A Casz1-NuRD complex regulates temporal identity transitions in neural progenitors. Sci Rep. 2021;11:3858 pubmed publisher
Shibata A, Ueda H, Eto K, Onda M, Sato A, Ohba T, et al. Photoactivatable CaMKII induces synaptic plasticity in single synapses. Nat Commun. 2021;12:751 pubmed publisher
Wei W, Riley N, Yang A, Kim J, Terrell S, Li V, et al. Cell type-selective secretome profiling in vivo. Nat Chem Biol. 2020;: pubmed publisher
Schohl A, Chorghay Z, Ruthazer E. A Simple and Efficient Method for Visualizing Individual Cells in vivo by Cre-Mediated Single-Cell Labeling by Electroporation (CREMSCLE). Front Neural Circuits. 2020;14:47 pubmed publisher
Zhang Y, Liu G, Guo T, Liang X, Du H, Yang L, et al. Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog. Cell Rep. 2020;30:4490-4504.e4 pubmed publisher
Tang Y, Zuniga Hertz J, Han C, Yu B, Cai D. Multifaceted secretion of htNSC-derived hypothalamic islets induces survival and antidiabetic effect via peripheral implantation in mice. elife. 2020;9: pubmed publisher
Kubo S, Murata C, Okamura H, Sakasegawa T, Sakurai C, Hatsuzawa K, et al. Oct motif variants in Beckwith-Wiedemann syndrome patients disrupt maintenance of the hypomethylated state of the H19/IGF2 imprinting control region. FEBS Lett. 2020;594:1517-1531 pubmed publisher
Guo J, Otis J, Suciu S, Catalano C, Xing L, Constable S, et al. Primary Cilia Signaling Promotes Axonal Tract Development and Is Disrupted in Joubert Syndrome-Related Disorders Models. Dev Cell. 2019;51:759-774.e5 pubmed publisher
Atasoy M, Rohaim M, Munir M. Simultaneous Deletion of Virulence Factors and Insertion of Antigens into the Infectious Laryngotracheitis Virus Using NHEJ-CRISPR/Cas9 and Cre-Lox System for Construction of a Stable Vaccine Vector. Vaccines (Basel). 2019;7: pubmed publisher
Frade J, Nakagawa S, Cortes P, Di Vicino U, Romo N, Lluis F, et al. Controlled ploidy reduction of pluripotent 4n cells generates 2n cells during mouse embryo development. Sci Adv. 2019;5:eaax4199 pubmed publisher
De León Reyes N, Mederos S, Varela I, Weiss L, Perea G, Galazo M, et al. Transient callosal projections of L4 neurons are eliminated for the acquisition of local connectivity. Nat Commun. 2019;10:4549 pubmed publisher
Sun Y, Espinosa J, Hoseini M, Stryker M. Experience-dependent structural plasticity at pre- and postsynaptic sites of layer 2/3 cells in developing visual cortex. Proc Natl Acad Sci U S A. 2019;116:21812-21820 pubmed publisher
Lin B. Modulation of the PTEN/mTOR pathway to enhance survival of cone photoreceptors in retinal degeneration disorders. Hong Kong Med J. 2019;25 Suppl 5:44-47 pubmed
Kim D, An H, Shearer R, Sharif M, Fan C, Choi J, et al. A principled strategy for mapping enhancers to genes. Sci Rep. 2019;9:11043 pubmed publisher
Tang P, Alex A, Nie J, Lee J, Roth A, Booth K, et al. Defective Tmprss3-Associated Hair Cell Degeneration in Inner Ear Organoids. Stem Cell Reports. 2019;: pubmed publisher
Zhang M, Ergin V, Lin L, Stork C, Chen L, Zheng S. Axonogenesis Is Coordinated by Neuron-Specific Alternative Splicing Programming and Splicing Regulator PTBP2. Neuron. 2019;101:690-706.e10 pubmed publisher
Lin B, Xiong G, Yang W. Ribosomal protein S6 kinase 1 promotes the survival of photoreceptors in retinitis pigmentosa. Cell Death Dis. 2018;9:1141 pubmed publisher
Benito N, Gaborieau E, Sanz Diez A, Kosar S, Foucault L, Raineteau O, et al. A Pool of Postnatally Generated Interneurons Persists in an Immature Stage in the Olfactory Bulb. J Neurosci. 2018;38:9870-9882 pubmed publisher
Fan L, Nehme R, Adam Y, Jung E, Wu H, Eggan K, et al. All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition. Nat Methods. 2018;15:823-831 pubmed publisher
Zhang P, Lu H, Peixoto R, Pines M, Ge Y, Oku S, et al. Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships. Cell. 2018;174:1450-1464.e23 pubmed publisher
Messier J, Chen H, Cai Z, Xue M. Targeting light-gated chloride channels to neuronal somatodendritic domain reduces their excitatory effect in the axon. elife. 2018;7: pubmed publisher
Al Batran R, Gopal K, Martin M, Ho K, Almutairi M, Aburasayn H, et al. Skeletal muscle-specific Cre recombinase expression, controlled by the human α-skeletal actin promoter, improves glucose tolerance in mice fed a high-fat diet. Diabetologia. 2018;61:1849-1855 pubmed publisher
de Melo J, Clark B, Venkataraman A, Shiau F, Zibetti C, Blackshaw S. Ldb1- and Rnf12-dependent regulation of Lhx2 controls the relative balance between neurogenesis and gliogenesis in the retina. Development. 2018;145: pubmed publisher
Zhou J, Benito Martin A, Mighty J, Chang L, Ghoroghi S, Wu H, et al. Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins. Sci Rep. 2018;8:2823 pubmed publisher
Roh Johnson M, Shah A, Stonick J, Poudel K, Kargl J, Yang G, et al. Macrophage-Dependent Cytoplasmic Transfer during Melanoma Invasion In Vivo. Dev Cell. 2017;43:549-562.e6 pubmed publisher
Meyer P, Maity P, Burkovski A, Schwab J, Müssel C, Singh K, et al. A model of the onset of the senescence associated secretory phenotype after DNA damage induced senescence. PLoS Comput Biol. 2017;13:e1005741 pubmed publisher
Londono R, Wenzhong W, Wang B, Tuan R, Lozito T. Cartilage and Muscle Cell Fate and Origins during Lizard Tail Regeneration. Front Bioeng Biotechnol. 2017;5:70 pubmed publisher
Remez L, Onishi A, Menuchin Lasowski Y, Biran A, Blackshaw S, Wahlin K, et al. Pax6 is essential for the generation of late-born retinal neurons and for inhibition of photoreceptor-fate during late stages of retinogenesis. Dev Biol. 2017;432:140-150 pubmed publisher
Koehler K, Nie J, Longworth Mills E, Liu X, Lee J, Holt J, et al. Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells. Nat Biotechnol. 2017;35:583-589 pubmed publisher
Azim K, Angonin D, Marcy G, Pieropan F, Rivera A, Donega V, et al. Pharmacogenomic identification of small molecules for lineage specific manipulation of subventricular zone germinal activity. PLoS Biol. 2017;15:e2000698 pubmed publisher
Briz C, Navarrete M, Esteban J, Nieto M. In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity. J Vis Exp. 2017;: pubmed publisher
Kuwajima T, Soares C, Sitko A, Lefebvre V, Mason C. SoxC Transcription Factors Promote Contralateral Retinal Ganglion Cell Differentiation and Axon Guidance in the Mouse Visual System. Neuron. 2017;93:1110-1125.e5 pubmed publisher
Xu J, Yao G, Ru Y, Xie S. Expression of tamoxifen-inducible CRE recombinase in Lcn5-CreERT2 transgenic mouse caput epididymis. Mol Reprod Dev. 2017;84:257-264 pubmed publisher
Lou S, Adam Y, Weinstein E, Williams E, Williams K, Parot V, et al. Genetically Targeted All-Optical Electrophysiology with a Transgenic Cre-Dependent Optopatch Mouse. J Neurosci. 2016;36:11059-11073 pubmed
de Melo J, Clark B, Blackshaw S. Multiple intrinsic factors act in concert with Lhx2 to direct retinal gliogenesis. Sci Rep. 2016;6:32757 pubmed publisher
Al Soudy A, Nakanishi T, Mizuno S, Hasegawa Y, Shawki H, Katoh M, et al. Germline recombination in a novel Cre transgenic line, Prl3b1-Cre mouse. Genesis. 2016;54:389-97 pubmed publisher
Lopez C, Saud K, Aguilar R, Berndt F, Cánovas J, Montecino M, et al. The chromatin modifying complex CoREST/LSD1 negatively regulates notch pathway during cerebral cortex development. Dev Neurobiol. 2016;76:1360-1373 pubmed publisher
de Melo J, Zibetti C, Clark B, Hwang W, Miranda Angulo A, Qian J, et al. Lhx2 Is an Essential Factor for Retinal Gliogenesis and Notch Signaling. J Neurosci. 2016;36:2391-405 pubmed publisher
Yuan M, Gao X, Chard L, Ali Z, Ahmed J, Li Y, et al. A marker-free system for highly efficient construction of vaccinia virus vectors using CRISPR Cas9. Mol Ther Methods Clin Dev. 2015;2:15035 pubmed publisher
Chen Y, Cao J, Xiong M, Petersen A, Dong Y, Tao Y, et al. Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Cell Stem Cell. 2015;17:233-44 pubmed publisher
Ochiai H, Sugawara T, Yamamoto T. Simultaneous live imaging of the transcription and nuclear position of specific genes. Nucleic Acids Res. 2015;43:e127 pubmed publisher
Xie C, Lu H, Nomura A, Hanse E, Forster C, Parker J, et al. Co-deleting Pten with Rb in retinal progenitor cells in mice results in fully penetrant bilateral retinoblastomas. Mol Cancer. 2015;14:93 pubmed publisher
Kearns N, Pham H, Tabak B, Genga R, Silverstein N, Garber M, et al. Functional annotation of native enhancers with a Cas9-histone demethylase fusion. Nat Methods. 2015;12:401-403 pubmed publisher
Ochiai H, Sugawara T, Sakuma T, Yamamoto T. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells. Sci Rep. 2014;4:7125 pubmed publisher
Xue M, Atallah B, Scanziani M. Equalizing excitation-inhibition ratios across visual cortical neurons. Nature. 2014;511:596-600 pubmed publisher
Zhao C, Farruggio A, Bjornson C, Chavez C, Geisinger J, Neal T, et al. Recombinase-mediated reprogramming and dystrophin gene addition in mdx mouse induced pluripotent stem cells. PLoS ONE. 2014;9:e96279 pubmed publisher
García Moreno F, Vasistha N, Begbie J, Molnar Z. CLoNe is a new method to target single progenitors and study their progeny in mouse and chick. Development. 2014;141:1589-98 pubmed publisher
Clarke S, Kang J, Ma D. The iFat1 transgene permits conditional endogenous n-3 PUFA enrichment both in vitro and in vivo. Transgenic Res. 2014;23:489-501 pubmed publisher
Azim K, Fischer B, Hurtado Chong A, Draganova K, Cantù C, Zemke M, et al. Persistent Wnt/β-catenin signaling determines dorsalization of the postnatal subventricular zone and neural stem cell specification into oligodendrocytes and glutamatergic neurons. Stem Cells. 2014;32:1301-12 pubmed publisher
Ageta Ishihara N, Miyata T, Ohshima C, Watanabe M, Sato Y, Hamamura Y, et al. Septins promote dendrite and axon development by negatively regulating microtubule stability via HDAC6-mediated deacetylation. Nat Commun. 2013;4:2532 pubmed publisher
Ryan S, Bonomo J, Zhao F, Cobb B. MHCII glycosylation modulates Bacteroides fragilis carbohydrate antigen presentation. J Exp Med. 2011;208:1041-53 pubmed publisher
Matsuda T, Cepko C. Controlled expression of transgenes introduced by in vivo electroporation. Proc Natl Acad Sci U S A. 2007;104:1027-32 pubmed
product information
Catalog Number :
13775
Product Name :
pCAG-Cre
article :
doi10.1073/pnas.0610155104
id1096
pubmed_id17209010
bacterial resistance :
Ampicillin
cloning :
backbonepCAGEN
backbone_mutation
backbone_origin
backbone_size4779
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Kozak consensus sequence was added before the start ATG. Myc-tag was added at the C-terminus of Cre.
growth strain :
Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer
origin :
37
pi :
alt_names
Cre recombinase
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5pCAG-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCre
shRNA_sequence
size1092
species
Bacteriophage P1
tags
locationC terminal on insert
tagMyc
plasmid copy :
The coding sequence of Cre was amplified by PCR using pxCANCre (Kanegae et al. NAR 23, 3816-3821 (1995)) obtained from Dr. Saito I (Univ. of Tokyo) as a template.
resistance markers :
71
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA