This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pScSR beta deltaN Flag
catalog :
13729
citations: 1
Reference
Schwartz T, Schmidt D, Brohawn S, Blobel G. Homodimerization of the G protein SRbeta in the nucleotide-free state involves proline cis/trans isomerization in the switch II region. Proc Natl Acad Sci U S A. 2006;103:6823-8 pubmed
product information
Catalog Number :
13729
Product Name :
pScSR beta deltaN Flag
article :
doi10.1073/pnas.0602083103
id1055
pubmed_id16627619
bacterial resistance :
Kanamycin
cloning :
backbonepET28a-derived
backbone_mutation
backbone_origin
backbone_size5332
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth temp :
Plasmid grows at 37, expression optimal at 30 degrees or 18 degrees
origin :
37
pi :
alt_names
SR
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5Nde1
promoter
sequencing_primer_3
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneSRP102
id853702
genbank_ids
mutationexpresses residues 31-244 of S. cerevisiae SR with a thrombin protease cleavable N-terminal 6xHis tag and a C-terminal Flag tag
nameSignal recognition particle receptor subunit
shRNA_sequence
size642
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tag6xHis
locationC terminal on backbone
tagFlag
resistance markers :
450
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA