This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCpSRb-5
catalog :
13727
citations: 1
Reference
Schwartz T, Schmidt D, Brohawn S, Blobel G. Homodimerization of the G protein SRbeta in the nucleotide-free state involves proline cis/trans isomerization in the switch II region. Proc Natl Acad Sci U S A. 2006;103:6823-8 pubmed
product information
Catalog Number :
13727
Product Name :
pCpSRb-5
article :
doi10.1073/pnas.0602083103
id1055
pubmed_id16627619
bacterial resistance :
Kanamycin
cloning :
backbonepET28a derived
backbone_mutation
backbone_origin
backbone_size5312
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Construct is expressed with a precission protease cleavable 6xHis tag
growth temp :
Plasmid grows at 37, expression optimal at 30 degrees or 18 degrees
origin :
37
pi :
alt_names
SR
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5Nde1
promoter
sequencing_primer_3T7 terminator
sequencing_primer_5T7 promoter
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneSRP102
id853702
genbank_ids
mutationSR circularly permuted D210E188 improved solubility from construct reported in paper
nameSignal recognition particle receptor subunit
shRNA_sequence
size591
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tag6xHis
resistance markers :
450
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA