pelB-MBP-RevA-ss | Addgene 137064 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pelB-MBP-RevA-ss

catalog :

137064

citations: 1

Reference
Robertson K, Truong C, Craciunescu F, Yang J, Chiu P, Fromme P, et al. Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi. Sci Rep. 2019;9:17606 pubmed publisher

product information

Catalog Number :

137064

Product Name :

pelB-MBP-RevA-ss

article :

doi	10.1038/s41598-019-53830-x
id	28207412
pubmed_id	31772280

bacterial resistance :

Kanamycin

cloning :

backbone

pelB-MBP (DNASU access no. EvNO00813783)

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli. RevA is a lipoprotein. RevA is not expected to be lipidated in this construct. Following TEV cleavage, RevA lacks 5 residues (CKAYV) at the N-terminus of the mature protein.

growth strain :

E. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

origin :

pi :

alt_names

RevA Bbm27 Bbp27 AAF07538.1

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	T7lac
sequencing_primer_3	GCTAGTTATTGCTCAGCGG
sequencing_primer_5	GAAAGGTGAAATCATGCCGAACATC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BB_P27
gene	revA
id	1194455

genbank_ids

mutation

lacks the RevA signal peptide and 5 residues (CKAYV) at the N-terminus of the mature protein

name

AAF07416.1

shRNA_sequence

size

species

32644

Other

Borrelia burgdorferi B31