pRevA-TEV-His12 | Addgene 137034 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRevA-TEV-His12

catalog :

137034

citations: 1

Reference
Robertson K, Truong C, Craciunescu F, Yang J, Chiu P, Fromme P, et al. Membrane directed expression in Escherichia coli of BBA57 and other virulence factors from the Lyme disease agent Borrelia burgdorferi. Sci Rep. 2019;9:17606 pubmed publisher

product information

Catalog Number :

137034

Product Name :

pRevA-TEV-His12

article :

doi	10.1038/s41598-019-53830-x
id	28207412
pubmed_id	31772280

bacterial resistance :

Ampicillin

cloning :

backbone

pRSET-TEV-12His

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

This plasmid uses the T7 promoter to express in E. coli the full-length, wild-type protein containing a C-terminal TEV-protease-cleavable His12-tag. The protein sequence includes its N-terminal membrane-targeting signal peptide sequence. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli.

growth strain :

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

origin :

pi :

alt_names

RevA Bbm27 Bbp27 AAF07416.1 AAF07538.1

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	T7
sequencing_primer_3	GCTAGTTATTGCTCAGCGG
sequencing_primer_5	TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BB_P27
gene	revA
id	1194455

genbank_ids

mutation

wild type, full-length protein, contains signal peptide

name

AAF07416.1

shRNA_sequence

size

species

32644

Other

Borrelia burgdorferi B31