This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTet-GLI2shR
catalog :
136691
citations: 1
Reference
Zhu H, Xia L, Shen Q, Zhao M, Gu X, Bouamar H, et al. Differential effects of GLI2 and GLI3 in regulating cervical cancer malignancy in vitro and in vivo. Lab Invest. 2018;98:1384-1396 pubmed publisher
product information
Catalog Number :
136691
Product Name :
pTet-GLI2shR
article :
doi10.1038/s41374-018-0089-5
id28200060
pubmed_id29967343
bacterial resistance :
Ampicillin
cloning :
The resulting pTet-GLI2shR plasmid was confirmed by single digestion with XhoI (Expected bands: 8447bp, 190bp, 136bp, 42bp)backbone_originbackbone_size8758promotersequencing_primer_3sequencing_primer_5vector_types
Lentiviral

(aattaaaaacctggcatgactaccactatgctcgagca
tagtggtagtcatgccagg).
backboneTet-pLKO-puro (Plasmid #21915)
backbone_mutationTet-pLKO-puro (Plasmid #21915) was digested with AgeI and EcoRI to insert Gli2 shRNA-coding sequence
growth strain :
3 )
CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGG
TAGTCATGCCAGGTTTTTG
Inducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5
origin :
37
pi :
sapiens
tags
THP2geneGLI2
id2736<
/tr>>genbank_ids>
NM_001371271
>mutationr>nameshRNA_humanGli2<
/td>shRNA_sequence>aattaaaaacctggcatgactaccactatgctcgagca
tagtggtagtcatgccaggsi
zespeciesd>
960
6
Homo
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5GGCAGGGATATTCACCATTATCGTTTCAGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCJS, HPE9, PHS2, THP1,
resistance markers :
2354
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA