This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.3-3XFLAG-AGO2
catalog :
136687
citations: 2
Reference
Briskin D, Wang P, Bartel D. The biochemical basis for the cooperative action of microRNAs. Proc Natl Acad Sci U S A. 2020;117:17764-17774 pubmed publisher
McGeary S, Lin K, Shi C, Pham T, Bisaria N, Kelley G, et al. The biochemical basis of microRNA targeting efficacy. Science. 2019;: pubmed publisher
product information
Catalog Number :
136687
Product Name :
pcDNA3.3-3XFLAG-AGO2
article :
doi10.1126/science.aav1741
id28207368
pubmed_id31806698
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.3
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please visit https://doi.org/10.1101/414763 for bioRxiv preprint.
growth strain :
Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5ATAATACCGCGCCACATAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10
geneAGO2
id27161
genbank_ids
27161
NM_012154.5
mutation
nameargonaute RISC Catalytic Component 2
shRNA_sequence
size2658
species
9606
Homo sapiens
tags
locationN terminal on insert
tag3XFLAG
resistance markers :
21
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA