This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBABE_hygro_mRFP1_NRF2(C506S, L4A)
catalog :
136580
citations: 1
Reference
Pereira E, Burns J, Lee C, Marohl T, Calderon D, Wang L, et al. Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies. Sci Signal. 2020;13: pubmed publisher
product information
Catalog Number :
136580
Product Name :
pBABE_hygro_mRFP1_NRF2(C506S, L4A)
article :
doi10.1126/scisignal.aba4200
id28207340
pubmed_id32291314
bacterial resistance :
Ampicillin
cloning :
backbonepBABE-hygro
backbone_mutation
backbone_originAddgene #1765
backbone_size5219
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEBP1, IMDDHH, NRF2, Nrf-2
geneNFE2L2
id4780
genbank_ids
NM_006164
mutationThe C506S mutation (C513S in the actual sequence of this plasmid) and leucine zipper (ZIP) mutation (L553A_L557A_L560A_L562A) were performed by site-directed mutagenesis of the pBabe mRFP1-Nrf2 hygro plasmid.
nameNRF2 (C506S, Leuzip mut)
shRNA_sequence
size2530
species
9606
Homo sapiens
tags
locationN terminal on insert
tagN-mRFP1-FLAG
resistance markers :
1585
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA