pSLIK_hygro_3xflag_NRF2(RR) | Addgene 136535 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSLIK_hygro_3xflag_NRF2(RR)

catalog :

136535

citations: 1

Reference
Pereira E, Burns J, Lee C, Marohl T, Calderon D, Wang L, et al. Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies. Sci Signal. 2020;13: pubmed publisher

product information

Catalog Number :

136535

Product Name :

pSLIK_hygro_3xflag_NRF2(RR)

article :

doi	10.1126/scisignal.aba4200
id	28207340
pubmed_id	32291314

bacterial resistance :

Ampicillin

cloning :

backbone

pSLIK-hygro

backbone_mutation

backbone_origin

Addgene #25737

backbone_size

13286

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

Other

destinatioin vector for gateway cloning

growth strain :

Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

origin :

pi :

sapienstags

location	N terminal on insert
tag	3x FLAG

ctggaaaatatagtagaactagagcaagatttcgttcgt
ttgaaagatgaaaaagaaaaattgctcaaa>nameNRF2(1584A>C,1586
A>T,1589A>G)shRNA_seq
uencesized>1815species>

9606

Homo

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HEBP1, IMDDHH, NRF2, Nrf-2
gene	NFE2L2
id	4780

genbank_ids

NM_006164

mutation

This plasmid is developed by mutating 3 base pairs within the sequence targeted by shNRF2 version 1 (c.1584A>C,c.1586A>T,c.1589A>G) of NRF2 sequence in the pEN_TTmiRc2_3xflag_NRF2 plasmid using QuickChange II XL site-directed mutagenesis kit to create an RNAi-resistant version of NRF2. These base pair changes do not change the amino acid sequence, but switch the codons to rare codons in the human genome. Primer used:

resistance markers :

1585

tags :

Unknown

terms :

Hygromycin