This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEN_TTmiRc2_p53(R280K)_V5
catalog :
136525
citations: 1
Reference
Pereira E, Burns J, Lee C, Marohl T, Calderon D, Wang L, et al. Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies. Sci Signal. 2020;13: pubmed publisher
product information
Catalog Number :
136525
Product Name :
pEN_TTmiRc2_p53(R280K)_V5
article :
doi10.1126/scisignal.aba4200
id28207340
pubmed_id32291314
bacterial resistance :
Gentamicin
cloning :
backbonepEN_TTmiRc2
backbone_mutation
backbone_originAddgene #25752
backbone_size4422
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
entry vector for gateway cloning
growth strain :
Used as a donor vector to clone into pSLIK
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3MfeI
cloning_site_5SpeI
promoter
sequencing_primer_3
sequencing_primer_5TGATAGAGAACGTATGTCGAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
NM_000546
mutationp53(R280K)V5 was cloned from pLenti6-p53-R280K-V5 plasmid from Addgene (plasmid #22933) with SpeI and MfeI 5' and 3' sites, respectively. There is a C --> G polymorphism at base pair 215 in ORF (corresponding to codon 72- switching from a proline to an arginine). This base pair substitution was encoded in the insert in the pLenti6 plasmid obtained from Addgene and is a common sequence polymorphism observed in p53.
namep53(R280K)
shRNA_sequence
size1278
species
9606
Homo sapiens
tags
locationC terminal on insert
tagV5
resistance markers :
1585
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA