pEN_TTmiRC2_3xflag_NRF2(RR) | Addgene 136522 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEN_TTmiRC2_3xflag_NRF2(RR)

catalog :

136522

citations: 2

Reference
Wang J, Wang X, Zhang M, Lang Y, Chen B, Ye Y, et al. The activation of spliced X-box binding protein 1 by isorhynchophylline therapy improves diabetic encephalopathy. Cell Biol Toxicol. 2023;: pubmed publisher
Pereira E, Burns J, Lee C, Marohl T, Calderon D, Wang L, et al. Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies. Sci Signal. 2020;13: pubmed publisher

product information

Catalog Number :

136522

Product Name :

pEN_TTmiRC2_3xflag_NRF2(RR)

article :

doi	10.1126/scisignal.aba4200
id	28207340
pubmed_id	32291314

bacterial resistance :

Gentamicin

cloning :

backbone

pEN_TT miRc2 3xFLAG

backbone_mutation

backbone_origin

Addgene #83274

backbone_size

4422

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

entry vector for gateway cloning

growth strain :

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

origin :

pi :

sapienstags

location	N terminal on insert
tag	3x FLAG

ctggaaaatatagtagaactagagcaagatttcgttcgt
ttgaaagatgaaaaagaaaaattgctcaaa>nameNRF2(1584A>C,1586
A>T,1589A>G)shRNA_seq
uencesized>1815species>

9606

Homo

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	MfeI
cloning_site_5	SpeI
promoter
sequencing_primer_3
sequencing_primer_5	actagtatggacttggagctgccg
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HEBP1, IMDDHH, NRF2, Nrf-2
gene	NFE2L2
id	4780

genbank_ids

NM_006164

mutation

This plasmid is developed by mutating 3 base pairs within the sequence targeted by shNRF2 version 1 (c.1584A>C,c.1586A>T,c.1589A>G) of NRF2 sequence in the pEN_TTmiRc2_3xflag_NRF2 plasmid using QuickChange II XL site-directed mutagenesis kit to create an RNAi-resistant version of NRF2. These base pair changes do not change the amino acid sequence, but switch the codons to rare codons in the human genome. Primer used:

resistance markers :

1585

tags :

Unknown