This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCEP4-PDGFRA-reporter
catalog :
136456
citations: 2
Reference
Yu Y, Alvarado R, Petty L, Bohlender R, Shaw D, Below J, et al. Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic cleft lip and palate. Hum Mol Genet. 2022;31:2348-2357 pubmed publisher
Park J, Gill K, Aghajani A, Heredia J, Choi H, Oberstein A, et al. Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. PLoS Pathog. 2020;16:e1008647 pubmed publisher
product information
Catalog Number :
136456
Product Name :
pCEP4-PDGFRA-reporter
article :
doi10.1371/journal.ppat.1008647
id28207324
pubmed_id32559251
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutation
backbone_originInvitrogen
backbone_size10375
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian fluorescent reporter plasmid for PDGFRA signaling.
origin :
37
pi :
alt_names
PDGFRA
platelet-derived growth factor receptor alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NheI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD140A, PDGFR-2, PDGFR2
genePDGFRA
id5156
genbank_ids
NP_006197.1
mutation
namePDGFRalpha
shRNA_sequence
size3324
species
9606
Homo sapiens
tags
locationN terminal on insert
tagSignal peptide from influenza HA
location
tagExtracellular c-myc epitope tag
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterMinimal promoter with artificial SRE enhancer.
sequencing_primer_3
sequencing_primer_5None
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameArray of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
shRNA_sequence
size1807
species
100
Synthetic
tags
locationC terminal on insert
tagThe PEST sequence from mouse ornithine decarboxylase (MODC) is fused to GFP to reduce its stability.
plasmid copy :
The minimal promoter and SRE enhancer were subcloned from Addgene plasmid 82686 (SRE reporter vector_559)
resistance markers :
3492
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA