This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-sPDGFRalpha-Fc-Y206S
catalog :
136439
citations: 1
Reference
Park J, Gill K, Aghajani A, Heredia J, Choi H, Oberstein A, et al. Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. PLoS Pathog. 2020;16:e1008647 pubmed publisher
product information
Catalog Number :
136439
Product Name :
pcDNA3-sPDGFRalpha-Fc-Y206S
article :
doi10.1371/journal.ppat.1008647
id28207324
pubmed_id32559251
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originInvitrogen
backbone_size5332
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)
origin :
37
pi :
alt_names
PDGFRA
platelet-derived growth factor receptor alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NheI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD140A, PDGFR-2, PDGFR2
genePDGFRA
id5156
genbank_ids
NP_006197.1
mutationExtracellular domain of PDGFR-alpha (a.a. 1-524) with Y206S mutation. Binds HCMV trimer, but no longer binds PDGFs.
namePDGFRalpha
shRNA_sequence
size2289
species
9606
Homo sapiens
tags
locationC terminal on insert
tagLinker GGGS
locationC terminal on insert
tagFc region of IgG1 (a.a. Asp-221 to Lys-447)
resistance markers :
3492
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA