This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3 PARL-FLAG-CT L79E
catalog :
13640
citations: 1
Reference
Sik A, Passer B, Koonin E, Pellegrini L. Self-regulated cleavage of the mitochondrial intramembrane-cleaving protease PARL yields Pbeta, a nuclear-targeted peptide. J Biol Chem. 2004;279:15323-9 pubmed
product information
Catalog Number :
13640
Product Name :
pcDNA3 PARL-FLAG-CT L79E
article :
doi10.1074/jbc.M313756200
id1119
pubmed_id14732705
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This mutation blocks PARL beta-cleavage without affecting the protein's rhomboid protease activity
origin :
37
pi :
alt_names
PARL
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5GTACGGTGGGAGGTCTATAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesPRO2207, PSARL, PSARL1, PSENIP2, RHBDS1
genePARL
id55486
aliasesPARL
genePARL
id460877
genbank_ids
AF197937
mutationchanged Leu 79 to Glu
namePresenilin-associated rhomboid-like
shRNA_sequence
size1164
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFLAG
resistance markers :
387
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA