This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET48 AtGUN1-PPR1
catalog :
136359
citations: 1
Reference
Shimizu T, Kacprzak S, Mochizuki N, Nagatani A, Watanabe S, Shimada T, et al. The retrograde signaling protein GUN1 regulates tetrapyrrole biosynthesis. Proc Natl Acad Sci U S A. 2019;116:24900-24906 pubmed publisher
product information
Catalog Number :
136359
Product Name :
pET48 AtGUN1-PPR1
article :
doi10.1073/pnas.1911251116
id28207309
pubmed_id31732672
bacterial resistance :
Kanamycin
cloning :
backbonepET48(+)
backbone_mutation
backbone_originNovagen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)
growth temp :
Use BL21 (DE3) for protein expression.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5EcoRI
promoterT7
sequencing_primer_3T7 reverse
sequencing_primer_5T7 forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G31400, T28P16.11, T28P16_11, genomes uncoupled 1
geneGUN1
id817698
genbank_ids
mutation
namecDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708
shRNA_sequence
size1428
species
3702
Arabidopsis thaliana
tags
locationN terminal on backbone
tagTRX-His
resistance markers :
4758
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA