This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFl Strep-sumo-TEV-hAgo2 D597N
catalog :
136236
citations: 1
Reference
Elkayam E, Parmar R, Brown C, Willoughby J, Theile C, Manoharan M, et al. siRNA carrying an (E)-vinylphosphonate moiety at the 5΄ end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2. Nucleic Acids Res. 2017;45:3528-3536 pubmed publisher
product information
Catalog Number :
136236
Product Name :
pFl Strep-sumo-TEV-hAgo2 D597N
article :
doi10.1093/nar/gkw1171
id28207277
pubmed_id27903888
bacterial resistance :
Ampicillin
cloning :
backbonepFl
backbone_mutation
backbone_origin
backbone_size6549
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Expressing catalytically inactive human Argonaute-2 in insect cells
origin :
37
pi :
alt_names
hAgo2
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterpolyhedrin
sequencing_primer_3MCS2 RV
sequencing_primer_5MCS2 FW
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10
geneAGO2
id27161
genbank_ids
mutationD597N
namehuman Argonaute-2
shRNA_sequence
size2580
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagSterp Sumo
resistance markers :
3340
tags :
High Copy
terms :
Gentamicin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA