pSLCAR-CD19-28z | Addgene 135991 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSLCAR-CD19-28z

catalog :

135991

citations: 2

Reference
Gong N, Han X, Xue L, El Mayta R, Metzloff A, Billingsley M, et al. In situ PEGylation of CAR T cells alleviates cytokine release syndrome and neurotoxicity. Nat Mater. 2023;22:1571-1580 pubmed publisher
Bloemberg D, Nguyen T, MacLean S, Zafer A, Gadoury C, Gurnani K, et al. A High-Throughput Method for Characterizing Novel Chimeric Antigen Receptors in Jurkat Cells. Mol Ther Methods Clin Dev. 2020;16:238-254 pubmed publisher

product information

Catalog Number :

135991

Product Name :

pSLCAR-CD19-28z

article :

doi	10.1016/j.omtm.2020.01.012
id	28207265
pubmed_id	32083149

bacterial resistance :

Ampicillin

cloning :

backbone

pSL

backbone_mutation

Use BpiI single pot restriction ligation to swap the antigen binding domain of the CAR from CD19 to desired specificity

backbone_origin

Heavily modified from LentiCRISPR v1 backbone

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth strain :

Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

origin :

pi :

alt_names

CD19(FMC63)-CAR, CD28 costim., CD3z stim domains, P2A-GFP marker

Contains: FMC63-scFV, Hinge domain, CD28-transmembrane domain, CD28-ITD, CD3z-ITD

cloning

clone_method	Restriction Enzyme
cloning_site_3	BpiI
cloning_site_5	BpiI
promoter	EF1a-short
sequencing_primer_3	caggagccTGCTCCTCTTACTcc
sequencing_primer_5	CGGGTTTGCCGCCA
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	Tp44
gene	CD28
id	940

aliases	CD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ
gene	CD247
id	919

genbank_ids

mutation

name

FMC63-28-3z-P2A-EGFP

shRNA_sequence

size

species

9606

Homo sapiens