This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-hRXR
catalog :
135910
citations: 1
Reference
Zolfaghari R, Mattie F, Wei C, Chisholm D, Whiting A, Ross A. CYP26A1 gene promoter is a useful tool for reporting RAR-mediated retinoid activity. Anal Biochem. 2019;577:98-109 pubmed publisher
product information
Catalog Number :
135910
Product Name :
pcDNA3.1-hRXR
article :
doi10.1016/j.ab.2019.04.022
id28207184
pubmed_id31039331
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutationNeomycin gene was removed and replaced with RXR at the sites of SmaI/BstBI.
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Neomycin gene was removed and replaced with RXR at the sites of SmaI/BstBI.
growth strain :
Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter
growth temp :
Follow the protocol from Promega
origin :
37
pi :
alt_names
RXR
cloning
clone_methodRestriction Enzyme
cloning_site_3BstBI
cloning_site_5SmaI
promoterSV40
sequencing_primer_3TATTCGAACTAAGTCATTTGGTGCGGCG
sequencing_primer_5GCCACCATGGACACCAAACATTTCCTG
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesNR2B1, RXR-alpha, RXRalpha
geneRXRA
id6256
genbank_ids
NM_002957
mutation
nameHuman retinoid X receptor- alpha
shRNA_sequence
size1389
species
9606
Homo sapiens
tags
resistance markers :
4742
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA