This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
catalog :
135766
citations: 1
Reference
Sanz Morejón A, Garcia Redondo A, Reuter H, Marques I, Bates T, Galardi Castilla M, et al. Wilms Tumor 1b Expression Defines a Pro-regenerative Macrophage Subtype and Is Required for Organ Regeneration in the Zebrafish. Cell Rep. 2019;28:1296-1306.e6 pubmed publisher
product information
Catalog Number :
135766
Product Name :
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
article :
doi10.1016/j.celrep.2019.06.091
id28207245
pubmed_id31365871
bacterial resistance :
Ampicillin
cloning :
backbonepDestTol2pA2AC
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
zebrafish
growth strain :
This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.
origin :
37
pi :
alt_names
Wt1b
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterUAS
sequencing_primer_3
sequencing_primer_5TTGAGCTCCTCCACACGAATTGAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasessi:ch211-160j6.3, wt1, zgc:152697
genewt1b
id568416
genbank_ids
mutation
nameWilms Tumor 1b gene (N-terminal domain)
shRNA_sequence
size792
species
7955
Danio rerio
tags
resistance markers :
3833
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA