This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFastBac SpyTag- 2AR-SpyTag
catalog :
135620
citations: 1
Reference
Choi H, Min D, Kang H, Shon M, Rah S, Kim H, et al. Watching helical membrane proteins fold reveals a common N-to-C-terminal folding pathway. Science. 2019;366:1150-1156 pubmed publisher
product information
Catalog Number :
135620
Product Name :
pFastBac SpyTag- 2AR-SpyTag
article :
doi10.1126/science.aaw8208
id28207233
pubmed_id31780561
bacterial resistance :
Ampicillin
cloning :
backbonepFastBac
backbone_mutationFlag tag on N terminus, eGFP-10xHis-R1D4 tag on C terminus have been added.
backbone_origin
backbone_size5693
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Expression of SpyTag-B2AR in insect cell
origin :
37
pi :
alt_names
B2AR
cloning
clone_methodRestriction Enzyme
cloning_site_3Nhe1
cloning_site_5Cla1
promoter
sequencing_primer_3CGT CGC CGT CCA GCT CGA CCA G
sequencing_primer_5GGA TTA TTC ATA CCG TCC CA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR
geneADRB2
id154
genbank_ids
mutationDeleted 1-24 aa, 366-413 aa
nameBeta 2 adrenergic receptor
shRNA_sequence
size1101
species
9606
Homo sapiens
tags
locationN terminal on insert
tagSpytag
locationC terminal on insert
tagSpytag
locationC terminal on backbone
tageGFP-10xHis-R1D4 tag
locationN terminal on backbone
tagHA-FLAG tag
resistance markers :
4740
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA