This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-28a(+)-FGF2-G3
catalog :
135521
citations: 1
Reference
Kuo H, Gao X, DeKeyser J, Fetterman K, Pinheiro E, Weddle C, et al. Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports. 2020;14:256-270 pubmed publisher
product information
Catalog Number :
135521
Product Name :
pET-28a(+)-FGF2-G3
article :
doi10.1016/j.stemcr.2019.12.007
id28197051
pubmed_id31928950
bacterial resistance :
Kanamycin
cloning :
backbonepET-28a (+)
backbone_mutation
backbone_originNovagen
backbone_size5369
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please visit https://www.biorxiv.org/content/10.1101/685503v2 for BioRxiv preprint
growth strain :
Engineered form of fibroblast growth factor 2 with improved thermostability
growth temp :
Use BL21 Star/DE3 for protein expression.
inserts :
BL21 Star/DE3
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterT7 Promoter
sequencing_primer_3GCT AGT TAT TGC TCA GCG G
sequencing_primer_5taatacgactcactataggg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBFGF, FGF-2, FGFB, HBGF-2
geneFGF2
id2247
genbank_ids
mutationCodon optimized for E. Coli production
namefibroblast growth factor 2
shRNA_sequence
size466
species
9606
Homo sapiens
tags
locationN terminal on insert
tag6x His tag
locationN terminal on insert
tagAgg Thrombin Tag
plasmid copy :
A portion of this plasmid was derived from a plasmid made by Printed sequence service from SGI. This plasmid contains the FGF2-G3 protein sequence which was first described by Dvorak et al. Biotechnol Bioeng 2018.
resistance markers :
4285
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA