This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRSET FLIPE intramol-1u
catalog :
13549
citations: 2
Reference
Pomorski A, Kochańczyk T, Miłoch A, Krezel A. Method for accurate determination of dissociation constants of optical ratiometric systems: chemical probes, genetically encoded sensors, and interacting molecules. Anal Chem. 2013;85:11479-86 pubmed publisher
Deuschle K, Okumoto S, Fehr M, Looger L, Kozhukh L, Frommer W. Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Protein Sci. 2005;14:2304-14 pubmed
product information
Catalog Number :
13549
Product Name :
pRSET FLIPE intramol-1u
article :
doi10.1110/ps.051508105
id968
pubmed_id16131659
bacterial resistance :
Ampicillin
cloning :
backbonepRSET-B
backbone_mutation
backbone_origin
backbone_size2897
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
For more information, see http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html
origin :
37
pi :
alt_names
ybeJ with ECFP insert
Glutamate/aspartate binding protein
gltI
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0655, ECK0648, JW5092, ybeJ, yzzK
genegltI
id946938
genbank_ids
NP_415188
mutationECFP insert in binding protein
nameFLIP-E intramol WT
shRNA_sequence
size2400
species
E. coli
tags
locationC terminal on insert
tagEYFP
resistance markers :
151
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA